MANF Antibody

Images

 
Western Blot: ARMET Antibody [NBP1-90099] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: ARMET Antibody [NBP1-90099] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human rectum shows moderate cytoplasmic positivity in peripheral ganglia, as well as glandular and lymphoid cells.
Immunohistochemistry-Paraffin: ARMET Antibody [NBP1-90099] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MANF Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREAR
Specificity
Specificity of human ARMET antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MANF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control
MANF Knockout 293T Cell Lysate
Control Peptide
Publications
Read Publication using NBP1-90099.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MANF Antibody

  • Arginine-Rich Protein
  • arginine-rich, mutated in early stage tumors
  • ARMET
  • ARP
  • MANF
  • mesencephalic astrocyte-derived neurotrophic factor
  • Protein ARMET

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MANF Antibody (NBP1-90099)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MANF Antibody (NBP1-90099) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for MANF Antibody (NBP1-90099)

Discover related pathways, diseases and genes to MANF Antibody (NBP1-90099). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on MANF

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Recombinant Monoclonal Antibodies

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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Bioinformatics

Gene Symbol MANF
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