MAML3 Antibody - BSA Free

Western Blot: MAML3 Antibody [NB100-2128] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2128] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2128). Detection: DAB

Immunoprecipitation: MAML3 Antibody [NB100-2128] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2128 used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by rabbit anti-MAML3 antibody NB100-2129. For blotting immunoprecipitated MAML3, NB100-2128 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MAML3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MAML3 Antibody - BSA Free (NB100-2128) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAML3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2- 10 ug/mg of lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAML3 Antibody - BSA Free

• CAG/CTG 3
• ERDA3
• MAM-2
• mam-3
• mastermind-like 3 (Drosophila)
• mastermind-like protein 3
• polyglutamine rich
• trinucleotide repeat containing 3

Background

The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. MAML3 acts as a transcriptional coactivator for NOTCH proteins and has been shown to amplify NOTCH-induced transcription of HES1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAML3
• Entrez:
  • 55534(Human)
• Uniprot:
  • NP_061187.2(Human)