MAML1 Antibody

Western Blot: MAML1 Antibody [NB100-1306] - (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MAML1 Antibody [NB100-1306] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MAML1 Antibody Summary

• Description: Novus Biologicals Goat MAML1 Antibody (NB100-1306) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MAML1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence VLPTCPMAEFALPR corresponding to N-Terminus according to NP_055572.1.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAML1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human skeletal muscle lysates (calculated MW of 108 kDa band according to NP_055572.1). IHC-P: Human placenta shows nuclear staining in trophoblasts.
• Control:

  Jurkat Whole Cell Lysate
  Human Ovary Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAML1 Antibody

• FLJ53540
• KIAA0200Mam1
• Mam-1mastermind homolog
• mastermind (drosophila)-like 1
• mastermind-like 1 (Drosophila)
• mastermind-like protein 1

Background

MAML1 is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAML1 Antibody (NB100-1306)(1)

Publication using NB100-1306

• Wu L, Aster JC, Blacklow SC et al. MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors. Nat Genet 2000-12-01 [PMID: 11101851]

Bioinformatics

• Gene Symbol: MAML1
• Entrez:
  • 9794(Human)
• Uniprot:
  • NP_055572.(Human)