MAMDC2 Antibody

Western Blot: MAMDC2 Antibody [NBP1-81169] - Analysis in control (vector only transfected HEK293T lysate) and MAMDC2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.

Immunocytochemistry/Immunofluorescence: MAMDC2 Antibody [NBP1-81169] - Staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: MAMDC2 Antibody [NBP1-81169] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: MAMDC2 Antibody [NBP1-81169] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: MAMDC2 Antibody [NBP1-81169] - Staining of human skin shows moderate positivity in plasma.

Immunohistochemistry-Paraffin: MAMDC2 Antibody [NBP1-81169] - Staining of human gastrointestinal shows moderate to strong cytoplasmic positivity in lymphoid cells.

Product Discontinued

MAMDC2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FSPVHCQNQTELLFSAVEASCNFEQDLCNFYQDKEGPGWTRVKVKPNMYRAGDHTTGLGYYLLANTKFTSQPGYIGRLYGPSLPGNLQYCLRFHYAIY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAMDC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MAMDC2 Antibody

• MAM domain containing 1
• MAM domain containing 2
• MAM domain-containing protein 2
• MAM domain-containing proteoglycan
• mamcan
• MGC21981

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAMDC2
• Entrez:
  • 256691(Human)