MALT1 Antibody (50) - BSA Free

Immunohistochemistry-Paraffin: MALT1 Antibody (50) [NBP1-43120] - Formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of Anti-Human MALT1 Purified (right) or Mouse IgG1 k Isotype Control Purified antibody (left) followed by Anti-Mouse Ig Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.

Product Discontinued

MALT1 Antibody (50) - BSA Free Summary

• Immunogen: The immunogen for this antibody was MALT1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MALT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This 50 antibody has been tested by immunohistochemistry on formalin-fixed paraffin-embedded (FFPE) human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at less than or equal to 10 ug/mL. This 50 antibody has been tested by western blotting under reducing conditions (at 5 ug/ml) on Jurkat cells.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for MALT1 Antibody (50) - BSA Free

• caspase-like protein
• DKFZp434L132
• EC 3.4.22.-
• MALT associated translocation
• MALT lymphoma-associated translocation
• MALT1
• MLT1
• MLTMALT-lymphoma associated translocation
• mucosa associated lymphoid tissue lymphoma translocation gene 1
• mucosa-associated lymphoid tissue lymphoma translocation protein 1
• Paracaspase

Background

The monoclonal antibody 50 recognizes human MALT1. MALT1 is a 91 kDa paracaspase that contains a death domain, two Ig-like domains and a C terminal caspase-like domain. Like other caspases, it cleaves substrates after an arginine residue. Expression is found in the cytoplasm of B and T cells. Within B cell follicles, MALT1 expression is highly expressed in centroblasts, followed by centrocytes and weakly expressed in the mantle zone. The interaction of MALT1 and Bcl10 occurs through Ig-like domains resulting in oligomerization and activation of the caspase-like domain. This also directly results in NFkappa B activation thereby playing a role in B cell maturation and activation. Rearrangements of the MALT1 gene found in t(1118)(q21q21) and t(1418)(q32q21) are the most frequent structural chromosomal abnormalities in MALT lymphomas. These translocations lead to fusions of API2(BIRC3)MALT1 and IGHMALT1 respectively. An additional translocation between MALT1 and MAP4 has also been identified in DLBCL (diffuse large B- cell lymphoma). The antibody 50 reacts with the C terminal domain of MALT1, which allows for the detection of endogenous MALT1 as well as both identified fusion proteins resulting from translocation events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MALT1