![Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Western-Blot-NBP2-41318-img0008.jpg "Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-P, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4. Amino Acid Squence: VGAQAPTTEEQEAAVS |
| Specificity | MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAGEA4 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of 293T cells transfected by pCMV6-ENTRY CTAG1B(RC213318) using anti-CTAG1B antibody (green, upper left; DAPI/blue, lower left; merged, upper right). 293T cells transfected with empty vector served as a negative control (merged, lower right). (1:100)](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Immunocytochemistry-Immunofluorescence-NBP1-47714-img0013.jpg)
![Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Flow-Cytometry-NBP1-47714-img0014.jpg)
![Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living NCI-H1299 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Flow-Cytometry-NBP1-47714-img0015.jpg)
![Flow (Intracellular): MAGE 1 Antibody (SPM282) - Azide and BSA Free [NBP2-34754] - An intracellular stain was performed on MG-63 cells with MAGE 1 Antibody [SPM282] NBP2-34754AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/images2/MAGE-1-Antibody-(SPM282)---Azide-and-BSA-Free-Flow-(Intracellular)-NBP2-34754-img0003.jpg)
![Flow (Intracellular): MAGE 1 Antibody (SPM282) - Azide and BSA Free [NBP2-34754] - An intracellular stain was performed on U2OS cells with MAGE 1 Antibody [SPM282] NBP2-34754AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 Azide and BSA Free format of this antibody.](http://images.novusbio.com/images2/MAGE-1-Antibody-(SPM282)---Azide-and-BSA-Free-Flow-(Intracellular)-NBP2-34754-img0004.jpg)
![Immunohistochemistry-Paraffin: MAGE 1 Antibody (SPM282) - Azide and BSA Free [NBP2-34754] - Formalin-paraffin human testis stained with MAGE-1 MAb (SPM282).](http://images.novusbio.com/images2/MAGE-1-Antibody-(SPM282)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34754-img0005.jpg)
![Western Blot: MAGEA6 Antibody [NBP3-05152] - Analysis of extracts of various cell lines, using MAGEA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/images2/MAGEA6-Antibody-Western-Blot-NBP3-05152-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAGEA6 Antibody [NBP3-05152] - Analysis of U2OS cells using MAGEA6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MAGEA6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05152-img0001.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Analysis in human testis and endometrium tissues using Anti-MAGEB2 antibody. Corresponding MAGEB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0001.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Staining of human testis shows high expression.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0002.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0003.jpg)
![Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining of human epididymis shows low expression as expected.](http://images.novusbio.com/images2/MAGEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85397-img0005.jpg)
![Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining in human testis and epididymis tissues using anti-MAGEC1 antibody. Corresponding MAGEC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MAGEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85397-img0007.jpg)
![Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining of human testis shows strong cytoplasmic positivity in spermatogonia.](http://images.novusbio.com/images2/MAGEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85397-img0008.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Analysis in human testis and prostate tissues. Corresponding CTAG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0008.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0009.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0010.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the PE/Cy5 conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP1-43073) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0014.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0013.jpg)
![Immunohistochemistry-Frozen: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis of Mouse colon cancer tissue using HLA ABC antibody (W6/32). Primary antibody dilution was 1:100. Sample was incubated with secondary antibody in 1:300 dilution. Image from verified customer review.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Immunohistochemistry-Frozen-NB100-64775-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0013.jpg)
![Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)](http://images.novusbio.com/images2/SCP1-Antibody-Western-Blot-NB300-229-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0015.jpg)
![Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-34145-img0006.jpg)
![Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human testis shows high expression.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-34145-img0007.jpg)
![Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining in human testis and endometrium tissues using anti-MAGEC2 antibody. Corresponding MAGEC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-34145-img0008.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0006.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0007.jpg)
![Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-(Intracellular)-NB200-103-img0003.jpg)
![ELISA: MAGEA12 Antibody (3A10) [H00004111-M01] - Detection limit for recombinant GST tagged MAGEA12 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-ELISA-H00004111-M01-img0003.jpg)
![Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in human liver.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-Western-Blot-H00004111-M01-img0007.jpg)
![Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in Raw 264.7.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-Western-Blot-H00004111-M01-img0004.jpg)
![Western Blot: GAGE1 Antibody [NBP2-54704] - Analysis in human cell line AN3-CA.](http://images.novusbio.com/images2/GAGE1-Antibody-Western-Blot-NBP2-54704-img0003.jpg)
![Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human testis shows strong nuclear positivity in subset of cells in seminiferious ducts.](http://images.novusbio.com/images2/GAGE1-Antibody-Immunohistochemistry-Paraffin-NBP2-54704-img0004.jpg)
![Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/images2/GAGE1-Antibody-Immunohistochemistry-Paraffin-NBP2-54704-img0005.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
Secondary Antibodies |
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Diseases for MAGEA4 Antibody (NBP2-41318)Discover more about diseases related to MAGEA4 Antibody (NBP2-41318).
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| Gene Symbol | MAGEA4 |
![Immunocytochemistry: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunocytochemistry-NBP2-41318-img0002.jpg "Immunocytochemistry: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunohistochemistry-Paraffin-NBP2-41318-img0007.jpg "Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0005.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - HeLa cells with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0006.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - HeLa cells with MAGEA4 antibody at 20 ug/ml.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0003.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.")
![Western Blot: MAGEA3 Antibody (6D10) [H00004102-M01] - Analysis of MAGEA3 expression in transfected 293T cell line by MAGEA3 monoclonal antibody (M01), clone 6D10.Lane 1: MAGEA3 transfected lysate (Predicted MW: 34.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/MAGEA3-Antibody-(6D10)-Western-Blot-H00004102-M01-img0005.jpg)
![Sandwich ELISA: MAGEA3 Antibody (6D10) [H00004102-M01] - Detection limit for recombinant GST tagged MAGEA3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/images2/MAGEA3-Antibody-(6D10)-Sandwich-ELISA-H00004102-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.](http://images.novusbio.com/images2/Melan-A-MART-1-Antibody-(A19-P)-Immunohistochemistry-Paraffin-NBP1-30151-img0011.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/Melan-A-MART-1-Antibody-(A19-P)-Immunohistochemistry-Paraffin-NBP1-30151-img0012.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")