![Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Western-Blot-NBP2-41318-img0008.jpg "Western Blot: MAGEA4 Antibody [NBP2-41318] - A431 cell lysate antibody at 1 ug/ml.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC, IHC-P, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4. The immunogen is located within the first 50 amino acids of MAGEA4. Amino Acid Squence: VGAQAPTTEEQEAAVS |
| Specificity | MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAGEA4 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for MAGEA4 Antibody (NBP2-41318)Discover more about diseases related to MAGEA4 Antibody (NBP2-41318).
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PTMs for MAGEA4 Antibody (NBP2-41318)Learn more about PTMs related to MAGEA4 Antibody (NBP2-41318).
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| Gene Symbol | MAGEA4 |
![Immunocytochemistry: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunocytochemistry-NBP2-41318-img0002.jpg "Immunocytochemistry: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in HeLa cells with MAGEA4 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunohistochemistry-Paraffin-NBP2-41318-img0007.jpg "Immunohistochemistry-Paraffin: MAGEA4 Antibody [NBP2-41318] - Human breast tumor with MAGEA4 antibody at 5 ug/mL.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0005.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - HeLa cells with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0006.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - HeLa cells with MAGEA4 antibody at 20 ug/ml.")
![Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/MAGEA4-Antibody-Immunofluorescence-NBP2-41318-img0003.jpg "Immunofluorescence: MAGEA4 Antibody [NBP2-41318] - Staining of MAGEA4 in human breast cancer tissue with MAGEA4 antibody at 20 ug/ml.")
![Western Blot: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3.](http://images.novusbio.com/images2/MAGEA3-Antibody-(1G9)-Western-Blot-NBP2-45719-img0003.jpg)
![Immunohistochemistry: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/MAGEA3-Antibody-(1G9)-Immunohistochemistry-NBP2-45719-img0002.jpg)
![Western Blot: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of extracts (10ug) from MCF7 cell line.](http://images.novusbio.com/images2/MAGEA3 Antibody (1G9)-Western Blot-NBP2-45719-img0004.jpg)
![Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Western-Blot-NBP1-47714-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of 293T cells transfected by pCMV6-ENTRY CTAG1B(RC213318) using anti-CTAG1B antibody (green, upper left; DAPI/blue, lower left; merged, upper right). 293T cells transfected with empty vector served as a negative control (merged, lower right). (1:100)](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Immunocytochemistry-Immunofluorescence-NBP1-47714-img0013.jpg)
![Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP1-47714, Dilution 1:50)](http://images.novusbio.com/images2/CTAG1B-Antibody-(OTI2B6)-Immunohistochemistry-Paraffin-NBP1-47714-img0018.jpg)
![Immunohistochemistry-Paraffin: MAGE 1 Antibody (MA454) [NBP2-29469] - Formalin-fixed, paraffin-embedded human testis stained with MAGE-1 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/images2/MAGE-1-Antibody-(MA454)-Immunohistochemistry-Paraffin-NBP2-29469-img0001.jpg)
![Western Blot: MAGEA6 Antibody [NBP3-05152] - Analysis of extracts of various cell lines, using MAGEA6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/images2/MAGEA6 Antibody-Western Blot-NBP3-05152-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAGEA6 Antibody [NBP3-05152] - Analysis of U2OS cells using MAGEA6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/MAGEA6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05152-img0001.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Staining of human liver.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0006.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Analysis in human testis and endometrium tissues using Anti-MAGEB2 antibody. Corresponding MAGEB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0001.jpg)
![Immunohistochemistry-Paraffin: MAGEB2 Antibody [NBP2-62688] - Staining of human testis shows high expression.](http://images.novusbio.com/images2/MAGEB2-Antibody-Immunohistochemistry-Paraffin-NBP2-62688-img0002.jpg)
![Western Blot: MAGEC1 Antibody [NBP1-85397] - Analysis in human cell line U-2 OS and human cell line CACO-2.](http://images.novusbio.com/images2/MAGEC1 Antibody-Western Blot-NBP1-85397-img0011.jpg)
![Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining in human testis and pancreas tissues.. Corresponding MAGEC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MAGEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85397-img0010.jpg)
![Immunohistochemistry-Paraffin: MAGEC1 Antibody [NBP1-85397] - Staining of human epididymis shows low expression as expected.](http://images.novusbio.com/images2/MAGEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85397-img0005.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0011.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Analysis in human testis and prostate tissues. Corresponding CTAG2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0008.jpg)
![Immunohistochemistry-Paraffin: CTAG2 Antibody [NBP2-49659] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/images2/CTAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-49659-img0009.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY licence.](http://images.novusbio.com/images2/OCT4-Antibody-Western-Blot-NB100-2379-img0014.jpg)
![Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.](http://images.novusbio.com/images2/OCT4-Antibody-Western-Blot-NB100-2379-img0011.jpg)
![Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/images2/OCT4-Antibody-Flow-Cytometry-NB100-2379-img0012.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Analysis using the FITC conjugate of NB100-64775. Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP1-43955) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0013.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0002.jpg)
![Flow Cytometry: HLA ABC Antibody (W6/32) [NB100-64775] - Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488.](http://images.novusbio.com/images2/HLA-ABC-Antibody-(W6-32)-Flow-Cytometry-NB100-64775-img0004.jpg)
![Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/SCP1-Antibody-Simple-Western-NB300-229-img0010.jpg)
![Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)](http://images.novusbio.com/images2/SCP1 Antibody-Western Blot-NB300-229-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human cell line SK-MEL-30 shows localization to nucleus & nucleoli.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34145-img0004.jpg)
![Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining in human testis and endometrium tissues using anti-MAGEC2 antibody. Corresponding MAGEC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-34145-img0008.jpg)
![Immunohistochemistry-Paraffin: Melanoma antigen family C2 Antibody [NBP2-34145] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/images2/Melanoma-antigen-family-C2-Antibody-Immunohistochemistry-Paraffin-NBP2-34145-img0006.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Confocal microscopy image of human fetal inner ear tisue section stained with Melan-A antibody (green), 1:200 dilution, incubated over night. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/Melan-A-MART-1-Antibody-(A19-P)-Immunohistochemistry-Paraffin-NBP1-30151-img0012.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A19-P) [NBP1-30151] - Human tissue (4 um section) stained with anti - Melan A monospecific clonal antibody.](http://images.novusbio.com/images2/Melan-A-MART-1-Antibody-(A19-P)-Immunohistochemistry-Paraffin-NBP1-30151-img0011.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in human liver.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-Western-Blot-H00004111-M01-img0007.jpg)
![Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in Raw 264.7.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-Western-Blot-H00004111-M01-img0004.jpg)
![Western Blot: MAGEA12 Antibody (3A10) [H00004111-M01] - MAGEA12 monoclonal antibody (M01), clone 3A10. Analysis of MAGEA12 expression in Jurkat.](http://images.novusbio.com/images2/MAGEA12-Antibody-(3A10)-Western-Blot-H00004111-M01-img0005.jpg)
![Western Blot: GAGE1 Antibody [NBP2-54704] - Analysis in human cell line AN3-CA.](http://images.novusbio.com/images2/GAGE1 Antibody-Western Blot-NBP2-54704-img0003.jpg)
![Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human testis shows strong nuclear and cytoplasmic positivity in spermatogonia.](http://images.novusbio.com/images2/GAGE1-Antibody-Immunohistochemistry-Paraffin-NBP2-54704-img0002.jpg)
![Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Flow-Cytometry-NBP1-49045-img0014.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")