MAD2L1 Antibody (2E2-1D6)

Western Blot: MAD2L1 Antibody (2E2-1D6) [H00004085-M01] - Analysis of MAD2L1 expression in transfected 293T cell line by MAD2L1 monoclonal antibody (M01), clone 2E2-1D6.Lane 1: MAD2L1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.

Western Blot: MAD2L1 Antibody (2E2-1D6) [H00004085-M01] - MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Analysis of MAD2L1 expression in HeLa.

Sandwich ELISA: MAD2L1 Antibody (2E2-1D6) [H00004085-M01] - Detection limit for recombinant GST tagged MAD2L1 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

MAD2L1 Antibody (2E2-1D6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAD2L1 (AAH00356, 1 a.a. ~ 205 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAD2L1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAD2L1 Antibody (2E2-1D6)

• HSMAD2
• MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• MAD2 mitotic arrest deficient-like 1 (yeast)
• MAD2L1
• MAD2-like protein 1
• MAD2mitotic arrest deficient, yeast, homolog-like 1
• Mitotic arrest deficient 2-like protein 1
• mitotic spindle assembly checkpoint protein MAD2A

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAD2L1 (H00004085-M01)(1)

Publication using H00004085-M01

• Yeh PC, Yeh CC, Chen YC, Juang YL. RED, a spindle pole-associated protein, is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint. J Biol Chem. 2012-02-18 [PMID: 22351768]

Bioinformatics

• Gene Symbol: MAD2L1