Recombinant Mouse M-CSF Protein

Product Discontinued

Recombinant Mouse M-CSF Protein Summary

• Description: Insect expressed C-terminal His-tagged Lys33-Pro187, Accession #: NM_001113530.

  Source: Insect

• Details of Functionality: The ED50 of this protein, as determined by M-NFS-60 proliferation, is less than or equal to 10 ng/ml. This corresponds to a specific activity of greater than or equal to 1 x 10^5Units/mg.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: CSF1
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37.79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) with 1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse M-CSF Protein

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

Macrophage colony-stimulating factor (M-CSF or CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. The secreted protein is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CSF1