M-CSF Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC217104 and based on accession number NM_000757. The protein contains a C-terminal DDK tag. |
| Specificity |
Homo sapiens colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1, mRNA. |
| Gene |
CSF1 |
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug). |
| Theoretical MW |
59.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
RIPA buffer |
Lysate Details for Array
| Type |
Overexpression |
| Protein State |
Native |
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for M-CSF Overexpression Lysate (Native)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Blogs on M-CSF
![Western Blot: MCSF Overexpression Lysate (Adult Normal) [NBL1-09513] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCSF.](http://images.novusbio.com/fullsize/MCSF Overexpression Lysate (Adult Normal)-Western Blot-NBL1-09513-img0002.jpg "Western Blot: MCSF Overexpression Lysate (Adult Normal) [NBL1-09513] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MCSF.")
![Western Blot: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Western blot analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/M-CSF-R-CD115-Antibody-(6B9H2)-Western-Blot-NBP2-37289-img0003.jpg)
![Immunohistochemistry-Paraffin: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/M-CSF-R-CD115-Antibody-(6B9H2)-Immunohistochemistry-Paraffin-NBP2-37289-img0007.jpg)
![Flow Cytometry: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Flow cytometric analysis of HepG2 cells using CSF1R mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/M-CSF-R-CD115-Antibody-(6B9H2)-Flow-Cytometry-NBP2-37289-img0004.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0017.jpg)
![Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Immunohistochemistry-Paraffin-NBP2-42388-img0023.jpg)
![Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.](http://images.novusbio.com/fullsize2/LAMC2-Antibody-(CL2980)-Western-Blot-NBP2-42388-img0015.jpg)
![Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver using 1:200 dilution of anti-Osteoprotegerin/TNFRSF11B antibody (clone 98A1071). The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Osteoprotegerin antibody generated a diffused but weak cytoplasmic staining with a very specific and strong punctate signal at the periphery of the cells.](http://images.novusbio.com/fullsize2/Osteoprotegerin-TNFRSF11B-Antibody-(98A1071)-Immunohistochemistry-Paraffin-NB100-56505-img0018.jpg)
![Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Osteoprotegerin in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Osteoprotegerin-TNFRSF11B-Antibody-(98A1071)-Western-Blot-NB100-56505-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Osteoprotegerin (98A1071) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Osteoprotegerin-TNFRSF11B-Antibody-(98A1071)-Immunocytochemistry-Immunofluorescence-NB100-56505-img0016.jpg)
![Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Western-Blot-NB100-56508-img0003.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0009.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control antibody red represents RANK antibody.](http://images.novusbio.com/fullsize2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0005.jpg){kind=icon}
![Functional: Recombinant Mouse M-CSF Protein [NBP1-43151] - Proliferation of M-NFS-60 cells in response to Mouse M-CSF Recombinant Protein.](http://images.novusbio.com/images2/Recombinant-Mouse-M-CSF-Protein-Functional-NBP1-43151-img0001.jpg "Functional: Recombinant Mouse M-CSF Protein [NBP1-43151] - Proliferation of M-NFS-60 cells in response to Mouse M-CSF Recombinant Protein.")
