Mouse M-CSF ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Csf1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Csf1. |
| Standard Curve Range |
31.2 to 2000 pg/mL |
| Sensitivity |
<1 pg/mL |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CSF1 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
| Publications |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- ABC diluent buffer
- Antibody diluent buffer
- Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
- Biotinylated anti-mouse M-CSF antibody, dilution 1:100
- Lyophilized recombinant mouse M-CSF standard
- One 96-well plate precoated with anti-mouse M-CSF antibody
- Sample diluent buffer
- TMB color developing agent
- TMB stop solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse M-CSF ELISA Kit (Colorimetric)
Background
M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for M-CSF ELISA Kit (KA0385)(3)
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Product General Protocols
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FAQs for M-CSF ELISA Kit (KA0385). (Showing 1 - 1 of 1 FAQs).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.