M-CSF Antibody

Product Discontinued

M-CSF Antibody Summary

• Immunogen: Recombinant full length mouse protein M-CSF, expressed in E.Coli.
• Localization: Secreted
• Specificity: Shows approximately 5% cross-reactivity with recombinant human M-CSF.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 0.1-1.0 ug/ml
  • Western Blot 0.5-1.0 ug/ml
• Application Notes: ELISA: Detect a limit of ~0.16 ng/well of recombinant mouse MCSF. Neut: Use at a concentration of 0.015 - 0.035 ug/ml to neutralize 50% of the bioactivity due to 2.5 ng/ml recombinant mouse MCSF using M-NFS-60 cells. WB: detect mouse M-CSF at 5 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 60 kDa.

Reactivity Notes

Cross-reacts with Mouse.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for M-CSF Antibody

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF1
• Entrez:
  • 1435(Human)
  • 12977(Mouse)