M-CSF Antibody (SU0413)

Western Blot: M-CSF Antibody (SU0413) [NBP2-67490] - Western blot analysis of M-CSF on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat

Immunocytochemistry/Immunofluorescence: M-CSF Antibody (SU0413) [NBP2-67490] - Staining MCSF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: M-CSF Antibody (SU0413) [NBP2-67490] - Analysis of paraffin-embedded human tonsil tissue using anti-MCSF antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: M-CSF Antibody (SU0413) [NBP2-67490] - Staining MCSF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: M-CSF Antibody (SU0413) [NBP2-67490] - Staining MCSF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: M-CSF Antibody (SU0413) [NBP2-67490] - Analysis of paraffin-embedded human liver tissue using anti-MCSF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: M-CSF Antibody (SU0413) [NBP2-67490] - Analysis of paraffin-embedded human lung tissue using anti-MCSF antibody. Counter stained with hematoxylin.

Product Discontinued

M-CSF Antibody (SU0413) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human M-CSF aa 56-105 / 554. (SwissProt: P09603 Human; SwissProt: P07141 Mouse)
• Localization: Membrane, Secreted, extracellular space, nuclear body.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for M-CSF Antibody (SU0413)

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

M-CSF (macrophage colony stimulating factor, colony stimulating factor 1, CSF-1) is a hemtopoietic growth factor produced by monocytes, granulocytes, endothelial cells, fibroblasts and activated B-cells and T-cells. M-CSF stimulates macrophage colony formation and regulates proliferation and differentiation of hematopoeitic stem cells into macrophages and regulates survival and differentiation of monocytes. It enhances antibody-dependent cell-mediated cytotoxity, enhances marophage killing of tumor cells and microorganisms, regulates the release of cytokines and inhibits bone resorption by osteoclasts. Human M-CSF shows biological activity on murine and rat cells and although murine M-CSF is active in rat cells it is inactive in human cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF1