M-CSF Antibody (26786) [Phycoerythrin]

Human macrophages treated with LPS and Recombinant Human TNF-a (210-TA) were stained with Mouse Anti-Human M-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

MG-63 cells were stained with Mouse Anti-Human M‑CSF PE‑conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 26786
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

M-CSF Antibody (26786) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human M-CSF
• Specificity: Detects human M-CSF in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSF Antibody (26786) [Phycoerythrin]

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSF Antibody (IC2161P)(1)

Publication using IC2161P

• M Tkach, J Thalmensi, E Timperi, P Gueguen, N Névo, E Grisard, P Sirven, F Cocozza, A Gouronnec, L Martin-Jau, M Jouve, F Delisle, N Manel, DC Rookhuizen, CL Guerin, V Soumelis, E Romano, E Segura, C Théry Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome Proceedings of the National Academy of Sciences of the United States of America, 2022-04-19;119(17):e2107394119. 2022-04-19 [PMID: 35439048] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CSF1