M-CSF Antibody (26786) [Allophycocyanin]

Human macrophages treated with LPS and Recombinant Human TNF-a (Catalog # 210-TA) were stained with Mouse Anti-Human M-CSF APC-conjugated Monoclonal Antibody (Catalog # IC2161A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 26786
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

M-CSF Antibody (26786) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human M-CSF
• Specificity: Detects human M-CSF.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSF Antibody (26786) [Allophycocyanin]

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF1