Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40049] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.128 ng/mL (example only; lot dependent)

Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.128 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KDM4A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A - Ready-To-Use ELISA Kit (Colorimetric)

• EC 1.14.11
• EC 1.14.11.-
• JHDM3A
• JHDM3AJMJD2
• JmjC domain-containing histone demethylation protein 3A
• JMJD2
• JMJD2A
• JMJD2Alysine-specific demethylase 4A
• jumonji domain containing 2
• jumonji domain containing 2A
• Jumonji domain-containing protein 2A
• KDM4A
• KIAA0677jumonji C domain-containing histone demethylase 3A
• Lysine (K)specific Demethylase 4A
• Lysine (K)-specific Demethylase 4A

Background

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A ELISA Kit (NBP3-40049). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KDM4A