Lysine Hydroxylase 2/PLOD2 Antibody

Images

 
Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of PLOD2 expression in human kidney.
Immunocytochemistry/ Immunofluorescence: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of purified antibody to PLOD2 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: Lysine Hydroxylase 2/PLOD2 Antibody [H00005352-B01P] - Analysis of PLOD2 expression in transfected 293T cell line by PLOD2 polyclonal antibody. Lane 1: PLOD2 transfected lysate(83.38 KDa). Lane 2: ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lysine Hydroxylase 2/PLOD2 Antibody Summary

Immunogen
PLOD2 (NP_891988.1, 1 a.a. - 758 a.a.) full-length human protein. MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP
Specificity
PLOD2 - procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PLOD2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.
Reviewed Applications
Read 1 Review rated 5
using
H00005352-B01P in the following applications:

Publications
Read Publications using
H00005352-B01P in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lysine Hydroxylase 2/PLOD2 Antibody

  • 2-oxoglutarate 5-dioxygenase (lysine hydroxylase) 2
  • 2-oxoglutarate 5-dioxygenase 2
  • LH2
  • Lysine Hydroxylase 2
  • PLOD2
  • procollagen-lysine
  • telopeptide lysyl hydroxylase
  • TLH

Background

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine Hydroxylase 2/PLOD2 Antibody (H00005352-B01P)(3)

Review for Lysine Hydroxylase 2/PLOD2 Antibody (H00005352-B01P) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00005352-B01P:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/22/2010
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Summary

ApplicationWestern Blot
Sample TestedMDA-231 cells, Sample Amount: 80
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk-TBS-T, Blocking Time: 30 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Incubation Dilution Buffer: 5% milk, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Mouse-HRP mAb (Santa Cruz), Secondary Ab Dilution Ratio: 1/2500

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 2 minutes, Specific Bands: ~90 kDa, Non-Specific Bands: no

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine Hydroxylase 2/PLOD2 Antibody (H00005352-B01P). (Showing 1 - 1 of 1 FAQs).

  1. I have been looking for anti PLOD2 antibody to perform IHC on human lung cancer tissue. I have tried some antibodies from different companies, but it did not work for us well. I found this antibody cat# H00005352-B01P at Novus Biologicals website. On the data sheet you reported it for ICC and IF. I have been using your different antibodies in the past and had some satisfying results. May I ask you, please, if you had tried IHC on human tissue and it did not work or you have not tried it at all?
    • H00005352-B01P is a product we distribute for a Taiwanese company called Abnova. We therefore do not do any in-house testing or development of their products. Abnova has validated this PLOD2 antibody for use in western via detection of the recombinant protein and overexpression transfected lysates. They have also validated this antibody in ICC with HeLa cells. They have not yet tested this antibody in tissue samples. See their datasheet here for all information we have regarding this product. Since this is not our product, we unfortunately cannot offer free samples. If you did want to try this product in an IHC experiment, we could offer you our Innovator's Reward Program. In exchange for a review of the product in an untested application or species, we would issue you a credit for the purchase price of the antibody for use in a future purchase. See details here Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Diseases for Lysine Hydroxylase 2/PLOD2 Antibody (H00005352-B01P)

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Recent Reviews

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5
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1
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Anonymous
01/22/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol PLOD2
Entrez
Uniprot