Lymphotoxin beta/TNFSF3 Antibody (135105)

• Reactivity: Hu
• Applications: WB
• Clone: 135105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Lymphotoxin beta/TNFSF3 Antibody (135105) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta
• Specificity: Detects human Lymphotoxin alpha beta heterotrimers in direct ELISAs and Western blots. This antibody detects an epitope in the Lymphotoxin beta subunit. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Lymphotoxin alpha , rhAPRIL, rhBAFF, rhTNF‑ alpha , rhTWEAK, rhEDA‑A2, rhEDA, rhTRAIL, rhVEGI, rhTRANCE, rhFas Ligand, or rhOX40 Ligand.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTB
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin beta/TNFSF3 Antibody (135105)

• LTB
• lymphotoxin beta (TNF superfamily, member 3)
• Lymphotoxin beta
• lymphotoxin-beta
• p33
• TNFCTNF-C
• TNFSF3
• TNFSF3LT-beta
• Tumor necrosis factor C
• Tumor necrosis factor ligand superfamily member 3

Background

Lymphotoxin beta is a type II transmembrane protein member of the TNF ligand superfamily and is now designated TNFSF3. Lymphotoxin beta associates with Lymphotoxin alpha to form two types of heterotrimers, Lymphotoxin alpha ₁ beta ₂ and Lymphotoxin  alpha ₂ beta ₁. Lymphotoxin beta is expressed by activated CD4 T cells, unpolarized IL‑2‑secreting effector T cells, and Th1 effector cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta/TNFSF3 Antibody (MAB1684)(2)

Publications using MAB1684

• Hashikawa K, Yasumoto S, Nakashima K, Arakawa F, Kiyasu J, Kimura Y, Saruta H, Nakama T, Yasuda K, Tashiro K, Kuhara S, Hashimoto T, Ohshima K Microarray analysis of gene expression by microdissected epidermis and dermis in mycosis fungoides and adult T-cell leukemia/lymphoma. Int J Oncol, 2014;45(3):1200-8. 2014 [PMID: 24970722] (IHC-P, Human)
• Young J, Yu X, Wolslegel K Lymphotoxin-alphabeta heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis. Cytokine, 2010;51(1):78-86. 2010 [PMID: 20356761] (Western Blot, Human)

Bioinformatics

• Gene Symbol: LTB