Lymphotoxin beta/TNFSF3 Antibody (135105) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 135105
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Lymphotoxin beta/TNFSF3 Antibody (135105) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta
• Specificity: Detects human Lymphotoxin alpha beta heterotrimers in direct ELISAs and Western blots. This antibody detects an epitope in the Lymphotoxin beta subunit. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Lymphotoxin alpha , rhAPRIL, rhBAFF, rhTNF‑ alpha , rhTWEAK, rhEDA‑A2, rhEDA, rhTRAIL, rhVEGI, rhTRANCE, rhFas Ligand, or rhOX40 Ligand.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTB
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lymphotoxin beta/TNFSF3 Antibody (135105) [CoraFluor™ 1]

• LTB
• lymphotoxin beta (TNF superfamily, member 3)
• Lymphotoxin beta
• lymphotoxin-beta
• p33
• TNFCTNF-C
• TNFSF3
• TNFSF3LT-beta
• Tumor necrosis factor C
• Tumor necrosis factor ligand superfamily member 3

Background

Tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-alpha, also known as TNF beta) are members of a family of secreted and cell surface cytokines that participate in the regulation of immune and inflammatory responses. LT-beta (lymphotaxin-beta or tumor necrosis factor C) is a type II membrane protein with significant homology to TNF, LT-alpha, and the ligand for the CD40 receptor. LT-alpha is present on the surface of activated T, B, and LAK cells as a complex with the 33 kda glycoprotein, LT-beta. LT-beta, also expressed by active lymphocytes, forms a heterotrimer with LT-a on the cell surface and anchors LT-alpha to the cell surface. A TNF receptor-related protein, the LT-beta receptor (also known as TNFC receptor), is the human receptor for the LT-alpha/LT-beta heterotrimer. There are two LT-beta isoforms expressed in human lymphoid cell lines and non-Hodgkin's lymphomas. The gene which encodes LT-beta maps to the major histocompatibility complex region on human chromosome 6p21.3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTB