Lymphotoxin beta R/TNFRSF3 Antibody (71319) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human Lymphotoxin beta R/TNFRSF3 PE-conjugated Monoclonal Antibody (Catalog # FAB629P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Expression of LT beta R and HVEM receptors on BEAS-2B cells.BEAS-2B cells and THP-1 cells (positive control) were treated with anti-PE labeled LT beta R antibody and anti-FITC labeled HVEM antibody. The fluorescence intensity was measured with a flow cytometer. THP-1 cells, a human monocytic cell line, were used as the positive control because they express both LT beta R and HVEM on their cell surface. The fluorescence intensities of LT beta R (A) and HVEM (B) suggest that BEAS-2B cells express LT beta R but not HVEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25501580), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 71319
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Lymphotoxin beta R/TNFRSF3 Antibody (71319) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
  Gln31-Met227
  Accession # P36941
• Specificity: Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) 4-1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, recombinant mouse (rm) Lymphotoxin beta R, rhNGF R, rhOPG, rhRELT, rhTAJ, rhTNF RI or rhTNF RII is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTBR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin beta R/TNFRSF3 Antibody (71319) [Phycoerythrin]

• CD18
• D12S370
• ltbetar
• LT-BETA-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

Lymphotoxin beta Receptor (LT beta R), also known as TNF RIII and TNF R-Related Protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric Lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
2. Rennert, P.D. et al. (1998) Immunity 9:71.
3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
5. Crowe, P.D. et al. (1994) Science 264:707.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta R/TNFRSF3 Antibody (FAB629P)(2)

Publications using FAB629P

• Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells. PLoS ONE, 2014-12-11;9(12):e114791. 2014-12-11 [PMID: 25501580] (Flow Cytometry, Human)
• DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1. BMC Cancer, 2012-05-14;12(0):176. 2012-05-14 [PMID: 22583667] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: LTBR
• Uniprot:
  • P36941()