Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Lymphotoxin‑ alpha /TNF‑ beta
• Specificity: Detects mouse Lymphotoxin-alpha /TNF-beta in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human Lymphotoxin-alpha /TNF-beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LTA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin-alpha/TNF-beta Antibody [Unconjugated]

• LT
• LTA
• LT-alpha
• lymphotoxin alpha (TNF superfamily, member 1)
• Lymphotoxin alpha
• Lymphotoxinalpha
• Lymphotoxin-alpha
• tnfb
• TNF-beta
• TNFBlymphotoxin-alpha
• TNFSF1
• TNFSF1B
• TNFSF1TNF-beta
• tumor necrosis factor beta
• Tumor necrosis factor ligand superfamily member 1

Background

Tumor necrosis factor-beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), is a secreted homotrimeric glycoprotein belonging to the TNF superfamily and is designated TNFSF1B. It is produced by NK, T, and B cells. TNF-beta was originally identified as protein that kills tumor cells in cell culture supernatants of a lymphoblastoid cell line. The TNF-beta subunit also associates with the type II transmembrane TNF superfamily protein lymphotoxin beta (LT beta ) to generate two types of heterotrimers designated as LT alpha 1 beta 2 (a single TNF-beta chain non-covalently associated with two chains of LT beta ), and LT alpha 2 beta 1 (1, 2). TNF-alpha , TNF-beta , and LT beta form a subfamily of the TNF related ligands. Their genes are genetically linked within a compact cluster inside the major histocompatibility complex locus (2, 3). The soluble TNF-beta binds and signals through TNF R1 and TNF R2. In contrast, the membrane-bound LT alpha 1 beta 2 interacts specifically with the LT beta receptor (LT beta R), which does not bind TNF-beta or TNF-alpha . Both TNFR1 and TNFR2 bind LT alpha 2 beta 1, which is recognized weakly by LT beta R (4, 5). TNF R1 and 2 express very broadly, while expression of LT beta R is restricted to stromal cells of lymphoid tissues. Herpesvirus entry mediator binds TNF-beta in vitro (6). The physiological importance of such interaction, if it occurs in vivo, is unclear. Distinct functions attributed to TNF-beta from transgenic knock-out mice include, loss of lymph node development, change in splenic architecture, impaired germinal center formation, and susceptibility to pulmonary tuberculosis (7, 8). TNF-beta also has overlapping physiological functions with LT beta and TNF-alpha in lymphoid organogenesis (7). Mouse and human TNF-beta share approximately 74% homology in their amino acid sequence.

1. Aggarwal, B.B. (2003) Nature Rev. Immunol. 3:745.
2. Browning , J.L. et al. (1993) Cell 72:846.
3. Browning, J.L. et al. (1995) J. Immunol. 154:33.
4. Pokholok, D.K. et al. (1995) Proc. Natl. Acad. Sci. USA 92:674.
5. Aggarwal, B.B. et al. (1985) Nature 318:665.
6. Crowe, P.D. et al. (1994) Science 264:707.
7. Mauri, D.N. et al. (1998) Immunity 8:21.
8. Tumanov, A.V. et al. (2003) Cytokine & Growth Factor Rev. 14:275.
9. Roach, D.R. et al. (2001) J. Exp. Med. 193:239.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin-alpha/TNF-beta Antibody (AF749)(1)

Publication using AF749

• Plant SR, Arnett HA, Ting JP Astroglial-derived lymphotoxin-alpha exacerbates inflammation and demyelination, but not remyelination. Glia, 2005-01-01;49(1):1-14. 2005-01-01 [PMID: 15382206] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: LTA