LY6G6D Antibody (3D6E10) - BSA Free

Flow Cytometry: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Jurkat cells using Gr-1/Ly-6G mouse mAb (green) and negative control (red).

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mouse mAb against Jurkat (1), MCF-7 (2), A549 (3),K562 (4),Hela (5),C6 (6),COS-7 (7),and NIH/3T3 (8) cell lysate.

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against HEK293-6e (1) and Gr-1/Ly-6G (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.

ELISA: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against human Gr-1/Ly-6G (AA: 20-104) recombinant protein. (Expected MW is 21.4 kDa)

Immunocytochemistry/Immunofluorescence: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Hela cells using Gr-1/Ly-6G mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3D6E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LY6G6D Antibody (3D6E10) - BSA Free Summary

• Description: Novus Biologicals Mouse Gr-1/Ly-6G Antibody (3D6E10) - BSA Free (NBP3-27164) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human LY6G6D (AA: 20-104) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY6G6D
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 13.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LY6G6D Antibody (3D6E10) - BSA Free

• C6orf23
• Chromosome 6 Open Reading Frame 23
• G6D
• LY6-D
• LY6G6D
• Lymphocyte Antigen 6 Complex Locus Protein G6d
• Lymphocyte Antigen 6 Complex, Locus G6D
• Lymphocyte Antigen-6 G6D
• Megakaryocyte-Enhanced Gene Transcript 1 Protein
• MEGT1
• NG25
• Protein Ly6-D

Background

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids, including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al., 2002 [PubMed 12079290]).[supplied by OMIM, Apr 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY6G6D