Ly-6G/Ly-6C Antibody (NIMP-R14) [PE]

Product Discontinued

Ly-6G/Ly-6C Antibody (NIMP-R14) [PE] Summary

• Description: Negative Control: Mouse Thymocytes
  Application Notes:
  IHC-F: Tissue was fixed with acetone
  IHC-P: Blocking with 20% normal rabbit serum
  FC: The recommended use of this reagent is 10ul per 250.000 cells in a 100ul total staining volume.
  FS: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection
• Immunogen: Purified BALB/c mouse neutrophils
• Epitope: This antibody recognizes a currently undetermined structure on the Neutrophil membrane
• Specificity: This monoclonal antibody is highly specific for murine Ly-6G and Ly-6C.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6g
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26831939).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.2um filtered antibody solution in PBS and 1.0% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Ly-6G/Ly-6C Antibody (NIMP-R14) [PE]

• GR
• Gr1
• Gr-1
• Ly-6
• Ly6G
• Ly-6G

Background

The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6G6C Antibody (NBP1-97923)(1)

Publication using NBP1-97923

• Zahr A, Alcaide P, Yang J et al. Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions. Nat Commun. 2016-02-02 [PMID: 26831939] (FLOW, Human)

FAQs for Ly-6G6C Antibody (NBP1-97923). (Showing 1 - 1 of 1 FAQ).

1. Does clone NIMP-R14 recognize both Ly-6G and Ly-6C or just Ly-6G?
   • This antibody recognizes both Ly-6G and Ly-6C.

Bioinformatics

• Gene Symbol: Ly6g