Ly-6G6C Antibody (NIMP-R14) [PE]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IA, IHC, IHC-Fr, IHC-P
Clone
NIMP-R14
Clonality
Monoclonal
Host
Rat

Order Details

Available Conjugates

Ly-6G6C Antibody (NIMP-R14) [PE] Summary

Immunogen
Purified BALB/c mouse neutrophils
Epitope
This antibody recognizes a currently undetermined structure on the Neutrophil membrane
Specificity
mouse Ly-6G6C
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LY6G6C
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
PE
Format
PE (A=488, E=575)
Buffer
0.2um filtered antibody solution in PBS and 1.0% BSA
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publication using
NBP1-97923 in the following applications:

Notes

Negative Control: Mouse Thymocytes
Application Notes:
IHC-F: Tissue was fixed with acetone
IHC-P: Blocking with 20% normal rabbit serum
FC: The recommended use of this reagent is 10ul per 250.000 cells in a 100ul total staining volume.
FS: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection

Alternate Names for Ly-6G6C Antibody (NIMP-R14) [PE]

  • C6orf24
  • G6c
  • lymphocyte antigen 6 complex locus protein G6c
  • lymphocyte antigen 6 complex, locus G6C
  • NG24
  • UNQ1947/PRO4430

Background

The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ly-6G6C Antibody (NBP1-97923)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Ly-6G6C Antibody (NBP1-97923). (Showing 1 - 1 of 1 FAQ).

  1. Does clone NIMP-R14 recognize both Ly-6G and Ly-6C or just Ly-6G?
    • This antibody recognizes both Ly-6G and Ly-6C.

Secondary Antibodies

 

Isotype Controls

Additional Ly-6G6C Antibody (NIMP-R14) [PE] Products

Ly-6G6C NBP1-97923

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Diseases for Ly-6G6C Antibody (NBP1-97923)

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Blogs on Ly-6G6C

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol LY6G6C

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