Ly-6G6C Antibody (NIMP-R14)

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Immunohistochemistry-Frozen: Ly-6G6C Antibody (NIMP-R14) [NB600-1387] - Staining for Ly-6G/-6C FITC of frozen section of mouse spleen.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, Func, IA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
NIMP-R14
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Ly-6G6C Antibody (NIMP-R14) Summary

Immunogen
Purified BALB/c mouse neutrophils
Epitope
This antibody recognizes a currently undetermined structure on the Neutrophil membrane
Specificity
mouse Ly-6G6C
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
LY6G6C
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NB600-1387 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26831939).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Ly-6G6C Antibody (NIMP-R14)

  • C6orf24
  • G6c
  • lymphocyte antigen 6 complex locus protein G6c
  • lymphocyte antigen 6 complex, locus G6C
  • NG24
  • UNQ1947/PRO4430

Background

Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ly-6G6C Antibody (NB600-1387)(23)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, ICC/IF, IHC, IHC-P.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC
(2)
IHC-P
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB600-1387 Applications Species
Zahr A, Alcaide P, Yang J et al. Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions. Nat Commun. 2016 Feb 02 [PMID: 26831939] (Flow, Human)

Details:
This citation used the PE version of this antibody.
Flow Human
Rosengarten M, Hadad N, Solomonov Y et al. Cytosolic phospholipase A2 a has a crucial role in the pathogenesis of DSS-induced colitis in mice. Eur. J. Immunol. 2015 Nov 09 [PMID: 26548451] (IHC-P, Mouse)

Details:
Ly-6G6C antibody (clone NIMP-R14) was used for IHC-P for the identification of neutrophils in paraffin processed colon sections. The IHC assay involved blocking of non-specific reactivity with 20% normal rabbit serum for 20 minutes, use of primary antibody at 1:10 dilution in PBS for 1 hour at RT and detection using 1:200 dilution of biotinylated anti-rat IgG secondary followed by avidin-biotin complex/HRP-DAB with hematoxylin counterstaining (Figure 4B).
IHC-P Mouse
Thangavel J, Malik AB, Elias HK et al. Combinatorial Therapy with Acetylation and Methylation Modifiers Attenuates Lung Vascular Hyperpermeability in Endotoxemia-Induced Mouse Inflammatory Lung Injury. Am. J. Pathol. 2014 Jun 12 [PMID: 24929240] (IHC, Mouse)

Details:
Ly-6G6C antibody used as neutrophil-specific marker antibody; Figure 7 B and C - IHC of lung tissues depicting reduced LPS-induced neutrophil recruitment into the airspace after a sublethal dose of LPS in mice treated with Aza+TSA (combination of DNA methyl transferase inhibitor 5-Aza 2-deoxycytidine and histone deacetylase inhibitor trichostatin A) compared with mice untreated and treated with Aza alone or TSA alone.
IHC Mouse
Hadad N, Burgazliev O, Elgazar-Carmon V et al. Induction of cytosolic phospholipase a2alpha is required for adipose neutrophil infiltration and hepatic insulin resistance early in the course of high-fat feeding. Diabetes. 2013 Sep [PMID: 23670971]
Kostarnoy AV, Gancheva PG, Logunov DY et al. Topical Bacterial Lipopolysaccharide Application Affects Inflammatory Response and Promotes Wound Healing J Interferon Cytokine Res. 2013 April 12 [PMID: 23581877]
Schmidt BA, Horsley V. Intradermal adipocytes mediate fibroblast recruitment during skin wound healing. Development 2013 Apr [PMID: 23482487] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Navarini AA, Lang KS, Verschoor A et al. Innate immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections Proc Natl Acad Sci USA [PMID: 19351895]

Details:
Used the FITC form of this antibody.
Song Y, Coleman L, Shi J et al. Inflammation, endothelial injury, and persistent pulmonary hypertension in heterozygous BMPR2-mutant mice. Am J Physiol Heart Circ Physiol;295(2):H677-90. 2008 Aug. [PMID: 18552156] (IHC-P, Mouse) IHC-P Mouse
Elgazar-Carmon V, Rudich A, Hadad N et al. Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding. J Lipid Res;49(9):1894-903. 2008 Sep. [PMID: 18503031] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF Mouse
Nagendra S, Schlueter AJ. Absence of cross-reactivity between murine Ly-6C and Ly-6G Cytometry A 2004 Apr [PMID: 15057973]

Details:
This citation used the FITC version of this antibody.
Show All 23 Publications.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Ly-6G6C Antibody (NB600-1387). (Showing 1 - 1 of 1 FAQs).

  1. I want to know whether the LY-6G6C Antibody(NIMP-R14) has alternate name,such as "Gr 1"
    • Our Ly-6G6C antibody with catalogue number NB600-1387 was raised against purified BALB/c mouse neutrophils, and is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins which includes Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight of between 15,000 and 18,000. Ly6G is together with Ly6C a component of the myeloid differentiation antigen Gr-1.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

FITC Labeled NBP1-60139
PE Labeled NBP1-97923

Additional Ly-6G6C Products

Ly-6G6C NB600-1387

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Bioinformatics

Gene Symbol LY6G6C