LXR alpha/NR1H3 Antibody (K8607)

YTHDF2 downregulates LXRA and HIVEP2 through m6A-dependent mRNA decay.a Western blotting of LXR alpha and HIVEP2 in GSC11 cells stably transfected with control shRNA or two YTHDF2 shRNAs. b Western blotting of LXR alpha and HIVEP2 in LN229/EGFRvIII tet-on cells cul Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33420027), licensed under a CC-BY license. Not internally tested by R&D Systems.

LXR alpha and HIVEP2 are functionally essential targets of YTHDF2 in cell proliferation, invasion, cholesterol dysregulation, and tumorigenesis of GBM cells. A Western blotting of YTHDF2, LXR alpha , and HIVEP2 in GSC11 cells stably expressing shCtrl, shYTHDF2, or shYTHDF2 plus shLXRA or/and shHIVEP2. Representative blot of three independent experiments is shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33420027), licensed under a CC-BY license. Not internally tested by R&D Systems.

YTHDF2 downregulates LXRA and HIVEP2 through m6A-dependent mRNA decay. b Western blotting of LXR alpha and HIVEP2 in LN229/EGFRvIII tet-on cells cultured with vehicle or Doxycycline and transfected with control siRNA or two individual YTHDF2 siRNAs Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33420027), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP, GS
• Clone: K8607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23015

LXR alpha/NR1H3 Antibody (K8607) Summary

• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR1H3

Applications/Dilutions

• Dilutions:
  • Gel Supershift Assay
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LXR alpha/NR1H3 Antibody (K8607)

• alpha
• Liver X receptor alpha
• liver X receptor-alpha
• LXR alpha
• LXRA
• NR1H3
• Nuclear receptor subfamily 1 group H member 3
• nuclear receptor subfamily 1, group H, member 3
• oxysterols receptor LXR-alpha
• RLD-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LXR alpha/NR1H3 Antibody (PP-K8607-00)(5)

Publications using PP-K8607-00

• Dong B, Kan C, Singh A, Liu J High-fructose diet downregulates long-chain acyl-CoA synthetase 3 expression in liver of hamsters via impairing LXR/RXR signaling pathway. J Lipid Res, 2013-02-20;54(5):1241-54. 2013-02-20 [PMID: 23427282] (Western Blot, Hamster)
• Minhao Wu, Bin Dong, Aiqin Cao, Hai Li, Jingwen Liu Delineation of molecular pathways that regulate hepatic PCSK9 and LDL receptor expression during fasting in normolipidemic hamsters Atherosclerosis 2012-10-01 [PMID: 22954675]
• Ronis MJ, Chen Y, Badeaux J, Badger TM Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J. Nutr., 2009-06-10;139(8):1431-8. 2009-06-10 [PMID: 19515742] (Western Blot, Rat)
• Delvecchio CJ, Capone JP Protein kinase C alpha modulates liver X receptor alpha transactivation. J. Endocrinol., 2008-04-01;197(1):121-30. 2008-04-01 [PMID: 18372238] (Western Blot, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Morello F, de Boer RA, Steffensen KR, Gnecchi M, Chisholm JW, Boomsma F, Anderson LM, Lawn RM, Gustafsson JK, Lopez-Ilasaca M, Pratt RE, Dzau VJ Liver X receptors alpha and beta regulate renin expression in vivo. J. Clin. Invest., 2005-07-01;115(7):1913-22. 2005-07-01 [PMID: 16007255] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: NR1H3