Lunapark Antibody - BSA Free

Western Blot: Lunapark Antibody [NBP1-80637] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Lunapark Antibody [NBP1-80637] - Staining of human cell line U-251 MG shows localization to nucleoplasm & endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human tonsil shows strong granular cytoplasmic positivity in germinal center cells.

Western Blot: Lunapark Antibody [NBP1-80637] - Analysis in human cell line U-138 MG.

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cervix shows moderate granular cytoplasmic positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lunapark Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lunapark Antibody - BSA Free (NBP1-80637) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Lunapark Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LNPK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Lunapark Protein (NBP1-80637PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lunapark Antibody - BSA Free

• 2310011O18Rik
• 4921514L11Rik
• DKFZp686G1442
• KIAA1715
• LNP
• Lunapark
• MGC102862
• protein lunapark
• Ul
• ulnaless

Background

KIAA1715 may be involved in limb and central nervous system development

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lunapark Antibody (NBP1-80637)(5)

Publications using NBP1-80637

• Tamara Moretti, Kyungho Kim, Astha Tuladhar, Jinoh Kim, Elizabeth Miller KLHL12 can form large COPII structures in the absence of CUL3 neddylation Molecular Biology of the Cell 2023-03-01 [PMID: 36652337]
• Hazra R, Brine L, Garcia L et al. Platr4 is an early embryonic lncRNA that exerts its function downstream on cardiogenic mesodermal lineage commitment Developmental cell 2022-11-07 [PMID: 36347239] (WB, Mouse)
• Akopian D, McGourty CA, RapE M Co-adaptor driven assembly of a CUL3 E3 ligase complex Molecular cell 2022-02-03 [PMID: 35120648] (WB, Human)
• P Bagchi, X Liu, WJ Cho, B Tsai Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape Cell Reports, 2021-12-07;37(10):110077. 2021-12-07 [PMID: 34879280] (Western Blot)
• de Almeida-Faria J, Duque-Guimar�es DE, Ong TP et al. Maternal obesity during pregnancy leads to adipose tissue ER stress in mice via miR-126-mediated reduction in Lunapark Diabetologia 2021-04-01 [PMID: 33501603] (Western Blot)

Bioinformatics

• Gene Symbol: LNPK
• Entrez:
  • 80856(Human)