Home » LSECtin/CLEC4G » CLEC4G Antibodies » LSECtin/CLEC4G Antibody (845404) [DyLight 594]

LSECtin/CLEC4G Antibody (845404) [DyLight 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
845404
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2947U-100UG
Alexa Fluor 405
FAB2947V-100UG
Alexa Fluor 488
FAB2947G-100UG
Alexa Fluor 532
FAB2947X
Alexa Fluor 594
FAB2947T-100UG
Alexa Fluor 647
FAB2947R-100UG
Alexa Fluor 700
FAB2947N-100UG
Alexa Fluor 750
FAB2947S-100UG
Allophycocyanin
FAB2947A
Biotin
FAB2947B
DyLight 350
FAB2947D
DyLight 405
FAB2947E
DyLight 488
FAB2947K
DyLight 550
FAB2947L
DyLight 650
FAB2947W
DyLight 680
FAB2947Y
DyLight 755
FAB2947Z
FITC
FAB2947F
Janelia Fluor 549
FAB2947I
Janelia Fluor 646
FAB2947J
PE
FAB2947P
PerCP
FAB2947C
Unconjugated
MAB2947-100
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2947-100
Carrier Free
FAB2947A
Carrier Free
MAB2947-SP
Carrier Free
MAB2947-100
Carrier Free
FAB2947V-100UG
Carrier Free
FAB2947U-100UG
Carrier Free
FAB2947T-100UG
Carrier Free
FAB2947S-100UG
Carrier Free
FAB2947R-100UG
Carrier Free
FAB2947N-100UG
Carrier Free
FAB2947G-100UG
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LSECtin/CLEC4G Antibody (845404) [DyLight 594] Summary

Immunogen
NS0 mouse myeloma cell line transfected with LSECtin/CLEC4G
Ser54-Cys293
Accession # Q6UXB4
Specificity
Detects human LSECtin/CLEC4G in flow cytometry.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CLEC4G
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for LSECtin/CLEC4G Antibody (845404) [DyLight 594]

  • CLEC4G
  • C-type lectin domain family 4 member G
  • C-type lectin domain family 4, member G
  • C-type lectin superfamily 4, member G
  • DTTR431
  • liver and lymph node sinusoidal endothelial cell C-type lectin
  • LP2698
  • LSECtin
  • Q6UXB4
  • UNQ431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DC-SIGN/CD209
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MAB161
Human DC‑SIGN and DC‑SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC‑SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
DC-SIGN was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(4 Reviews)
(40 Publications)
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, WB
CD23/Fc epsilon
RII Antibody [Unconju ...
AF123
CD23/Fc epsilon  RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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(2 Publications)
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Applications: Block, ICC, WB
IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
(3 Publications)
Species: Hu
Applications: BA
RNF130 Antibody
NBP1-81858
Immunohistochemistry-Paraffin: RNF130 Antibody [NBP1-81858] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Western Blot: RNF130 Antibody [NBP1-81858] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-478
Species: Hu
Applications: IHC, IHC-P, WB
Tenascin C
Antibody (4C8MS) - BS
NB110-68136
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
(2 Reviews)
(24 Publications)
Species: Fe, Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Axl [p Tyr779]
Antibody
AF2228
Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho‑Axl (Y779) Antigen Affinity‑purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho‑Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was detected using Rabbit Anti-Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D); Catalog # NL004) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C); Catalog # NL003). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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SP2 Antibody
NBP1-83054
Immunocytochemistry/Immunofluorescence: SP2 Antibody [NBP1-83054] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SP2 Antibody [NBP1-83054] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Dystroglycan
Antibody
NBP2-14868
Western Blot: Dystroglycan Antibody [NBP2-14868] - U87-MG whole cell extracts and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Dystroglycan Antibody [NBP2-14868] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dystroglycan protein stained by Dystroglycan antibody 38) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Dystroglycan Antibody [NBP2-14868] - Mouse placenta. DAG1 stained by Dystroglycan antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
(2 Publications)
Species: Eq, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PU.1/Spi-1 Antibody
NBP2-27163
Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.
Western Blot: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Analysis of PU.1/Spi1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27163.
Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control this antibody ; red represents anti-PU.1 antibody.
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c-Myb Antibody
NBP1-80306
Western Blot: c-Myb Antibody [NBP1-80306] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry-Paraffin: c-Myb Antibody [NBP1-80306] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: c-Myb Antibody [NBP1-80306] - WB Suggested Anti-MYB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate
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RUNX3/CBFA3
Antibody (527327) [Un ...
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RUNX3/CBFA3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse RUNX3/CBFA3 Monoclonal Antibody (Catalog # MAB3765) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse RUNX3/CBFA3 Monoclonal Antibody (Catalog # MAB3765) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RUNX3/CBFA3 at approximately 48 and 52  kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for RUNX3/CBFA3 at approximately 54 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse RUNX3/CBFA3 Monoclonal Antibody (Catalog # MAB3765). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
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ARC/NOL3 Antibody
NBP2-19561
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NOL3 stained by NOL3 antibody diluted at 1:500.
Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Mouse brain. NOL3 stained by NOL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD68/SR-D1
Antibody (KP1) - BSA
NB100-683
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68/SR-D1 Antibody NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1:50 for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody.
Flow Cytometry: CD68/SR-D1 Antibody (KP1) [NB100-683] - An intracellular stain was performed on THP-1 cells with CD68 Antibody [KP1] NB100-683 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (KP1) [NB100-683] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD68 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
(1 Review)
(51 Publications)
Species: Hu, Mu, Rt
Applications: Dual ISH-IHC, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Dtk/TYRO3 Antibody
(96201) [Unconjugated ...
MAB859
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Tyro3/Dtk knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human Tyro3/Dtk Monoclonal Antibody (Catalog # MAB859, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Tyro3/Dtk knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
(8 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, KO
RPGR Antibody
NBP1-84412
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RPGR Antibody [NBP1-84412] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
(2 Publications)
Species: Hu
Applications: IHC, IHC-P
CD28 Antibody
(37407) [Unconjugated
MAB342
Human CD28 Monoclonal Antibody enhances IL-2 secretion in the Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.2-0.6 μg/mL.
(2 Reviews)
(33 Publications)
Species: Hu
Applications: AgAct, WB
FANCC Antibody
NBP1-18979
Western Blot: FANCC Antibody [NBP1-18979] - Western blot analysis of FANCC expression in 1) NTera2 and 2) Cos 7 whole cell lysates.
Western Blot: FANCC Antibody [NBP1-18979] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2. anti-FANCC [NBP1-18979].
Species: Hu, Pm
Applications: Flow, ICC/IF, IP, WB
CRX/CORD2 Antibody
(4G11)
H00001406-M02
Western Blot: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M02), clone 4G11.Lane 1: CRX transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Retinal development is recapitulated in differentiating stem cells. Immunostaining of day 49 cultures shows remnants of retinal organization. CRX+ photoreceptor progenitors in green and mCherry+ RGCs in magenta. CRX+ cells - white arrows - appear to segregate from mCherry+ axons, suggesting division between the outer nuclear layer and the nerve fiber layer. Scale bar?=?500?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep16595), licensed under a CC-BY licence.
ELISA: CRX/CORD2 Antibody (4G11) [H00001406-M02] - Detection limit for recombinant GST tagged CRX is approximately 0.03ng/ml as a capture antibody.
(19 Publications)
Species: Bv, Fi, Hu, Mu
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
SDSL Antibody
(OTI1A8) - Azide and ...
NBP2-74045
Western Blot: SDSL Antibody (OTI1A8) - Azide and BSA Free [NBP2-74045] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDSL.
Flow Cytometry: SDSL Antibody (OTI1A8) - Azide and BSA Free [NBP2-74045] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by SDSL antibody.
Immunohistochemistry: SDSL Antibody (OTI1A8) - Azide and BSA Free [NBP2-74045] - Analysis of Carcinoma of Human thyroid tissue.
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB

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Bioinformatics

Gene Symbol CLEC4G