LSECtin/CLEC4G Antibody (845404) [Allophycocyanin]

Human mature dendritic cells were stained with Mouse Anti-Human LSECtin/CLEC4G APC-conjugated Monoclonal Antibody (Catalog # FAB2947A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 845404
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

LSECtin/CLEC4G Antibody (845404) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LSECtin/CLEC4G
  Ser54-Cys293
  Accession # Q6UXB4
• Specificity: Detects human LSECtin/CLEC4G in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC4G
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LSECtin/CLEC4G Antibody (845404) [Allophycocyanin]

• CLEC4G
• C-type lectin domain family 4 member G
• C-type lectin domain family 4, member G
• C-type lectin superfamily 4, member G
• DTTR431
• liver and lymph node sinusoidal endothelial cell C-type lectin
• LP2698
• LSECtin
• Q6UXB4
• UNQ431

Background

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca²⁺-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1‑31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca²⁺-dependent carbohydrate‑recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca²⁺-dependent manner (1‑3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin‑mediated infection of cells by Ebola virus has been demonstrated (3, 4).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.
3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.
4. Gramberg, T. et al. (2005) Virology 340:224.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LSECtin/CLEC4G Antibody (FAB2947A)(4)

Publications using FAB2947A

• D Avancini, A Testori, L Fresolone, G Andolfi, M Vuono, V Martinelli, FR Santoni de, S Gregori Aryl hydrocarbon receptor activity downstream of IL-10 signaling is required to promote regulatory functions in human dendritic cells Cell Reports, 2023-03-03;42(3):112193. 2023-03-03 [PMID: 36870061] (Flow Cytometry, ICC, Human)
• Barrett TJ, Bilaloglu S, Cornwell M et al. Platelets contribute to disease severity in COVID-19 Journal of thrombosis and haemostasis : JTH 9/19/2021 [PMID: 34538015]
• KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman Identification of HIV transmitting CD11c+ human epidermal dendritic cells Nat Commun, 2019-06-21;10(1):2759. 2019-06-21 [PMID: 31227717] (Flow Cytometry, Human)
• RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes J. Leukoc. Biol, 2017-03-07;0(0):. 2017-03-07 [PMID: 28270408] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLEC4G
• Uniprot:
  • Q6UXB4()