LRRK2 Antibody - BSA Free

Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: LRRK2 Antibody [NB110-58771] - NB110-58771 in increasing amounts of human lymphoblast

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB110-58771] - Immunofluorescence of Lrrk2 with NB110-58771 within (A) Substantia nigra neurons (B) Chains of migrating neuroblasts.

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.

Western Blot: LRRK2 Antibody [NB110-58771] - LRRK2 RNA levels 5 days after shRNA lentiviral transduction. The empty vector was used as the mock control. Lines represent the mean +/- SEM of three to four independent silencing experiments. A representative immunoblot analysis of LRRK2 protein levels 5 days after transduction is also shown. Beta-III-tubulin was the loading control. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x) licensed under a CC-BY license.

Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( beta III-tubulin, Tau, & alpha -synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with beta III-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha -tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF beta III transforming growth factor beta III, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0761-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Po
• Applications: WB, Flow, ICC/IF, IHC, IP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LRRK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit LRRK2 Antibody - BSA Free (NB110-58771) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-LRRK2 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal region, within residues 2500-2527 of the human LRRK2 protein, conjugated to KLH. [Swiss-Prot# Q5S007]
• Localization: Cytoplasm. Membrane; Peripheral membrane protein.
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) reported in scientific literature (PMID 20483355)
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 24312256)
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:1000
• Theoretical MW: 286 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRRK2 Antibody - BSA Free

• augmented in rheumatoid arthritis 17
• AURA17
• Dardarin
• DKFZp434H2111
• EC 2.7.11.1
• FLJ45829
• leucine-rich repeat kinase 2
• leucine-rich repeat serine/threonine-protein kinase 2
• LRRK2
• PARK8
• PARK8DARDARIN
• Parkinson disease (autosomal dominant) 8
• RIPK7
• ROCO2

Background

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRK2 Antibody (NB110-58771)(8)

Publications using NB110-58771

• Labib M, Wang Z, Kim Y et al. Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting Nature biomedical engineering 2023-11-27 [PMID: 38012306]
• Lopez de Maturana R, Lang V, Zubiarrain A et al. Mutations in LRRK2 impair NF-kB pathway in iPSC-derived neurons. J Neuroinflammation. 2016-11-18 [PMID: 27863501] (WB, Human)
• Skibinski G, Nakamura K, Cookson MR, Finkbeiner S. Mutant LRRK2 toxicity in neurons depends on LRRK2 levels and synuclein but not kinase activity or inclusion bodies. J Neurosci. 2014-01-08 [PMID: 24403142] (ICC/IF)
• Nakamori M, Takahashi T, Nishikawa T et al. Molecular Markers for Granulovacuolar Degeneration Are Present in Rimmed Vacuoles. PLoS One. 2013-11-28 [PMID: 24312256] (IHC-P, ICC/IF, Human)
• Melrose HL, Dachsel JC, Behrouz B et al. Impaired dopaminergic neurotransmission and microtubule-associated protein tau alterations in human LRRK2 transgenic mice. Neurobiol Dis 2010-12-01 [PMID: 20659558] (WB, Mouse)
• Lee H, Melrose HL, Yue M et al. Lrrk2 localization in the primate basal ganglia and thalamus: a light and electron microscopic analysis in monkeys. Exp Neurol;224(2):438-47. 2010-08-01 [PMID: 20483355] (IF/IHC, WB, ICC/IF, Primate)
• Melrose H. Update on the functional biology of Lrrk2. Future Neurol. 2008-01-01 [PMID: 19225574] (WB, Mouse)
• Melrose, HL et al. A comparative analysis of leucine-rich repeat kinase 2 (Lrrk2) expression in mouse brain Lewy Body disease. Neurosci 147: 1047-1058. 2007-01-01 [PMID: 17611037] (IF/IHC, WB, Mouse)

FAQs for LRRK2 Antibody (NB110-58771). (Showing 1 - 1 of 1 FAQs).

1. Is the immunogen of NB110-58771 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?
   • The immunogen used to generate NB110-58771 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt # Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.

Bioinformatics

• Gene Symbol: LRRK2