LRPAP Antibody

Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse LRPAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRPAP at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRPAP Antibody Summary

• Immunogen: E. coli-derived recombinant mouse LRPAP
  Gln29-Leu360
  Accession # P55302
• Specificity: Detects mouse LRPAP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human LRPAP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRPAP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRPAP Antibody

• A2MRAP
• A2MRAPMRAP
• A2RAP
• alpha-2-macroglobulin receptor-associated protein 1
• alpha-2-macroglobulin receptor-associated protein
• alpha-2-MRAP
• HBP44
• lipoprotein receptor associated protein
• low density lipoprotein receptor-related protein associated protein 1
• Low density lipoprotein receptor-related protein-associated protein 1
• low density lipoprotein-related protein-associated protein 1(alpha-2-macroglobulin receptor-associated protein 1)
• LRPAP
• LRPAP1
• MGC138272
• RAP

Background

LRPAP (LDL receptor-related protein-associated protein 1; also named RAP) is a ubiquitously expressed 39 kDa molecular chaperone for LDL receptor family proteins (1, 2). Mature mouse LRPAP is 332 amino acids (aa) in length and secreted into the ER/Golgi of the cell. It shares 77% and 97% aa sequence identity with human and rat LRPAP, respectively. LRPAP contains three approximately 100 aa alpha -helical domains (D1‑D3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). Domains D2 and D3 interact with each other, while D1 is independent (3). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their co-expressed ligands, thereby promoting proper receptor folding and export from the ER (6‑8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10‑14).

1. Furukawa, T. et al. (1990) J. Biochem. 108:297. 
2. Bu, G. (2001) Int. Rev. Cytol. 209:79. 
3. Lazic, A. et al. (2003) Biochemistry 42:14913. 
4. Lazic, A. et al. (2006) Arch. Biochem. Biophys. 450:167. 
5. Bu, G. et al. (1994) J. Biol. Chem. 269:29874. 
6. Willnow, T.E. et al. (1996) EMBO J. 15:2632. 
7. Bu, G. and S. Rennke (1996) J. Biol. Chem. 271:22218.
8. Obermoeller, L.M. et al. (1997) J. Biol. Chem. 272:10761.
9. Lee, D. et al. (2006) Mol. Cell 22:423.
10. Williams, S.E. et al. (1992) J. Biol. Chem. 267:9035.
11. Medh, J.D. et al. (1995) J. Biol. Chem. 270:536.
12. Herz, J. et al. (1991) J. Biol. Chem. 266:21232.
13. Mokuno, H. et al. (1994) J. Biol. Chem. 269:13238.
14. Orth, K. et al. (1992) Proc. Natl. Acad. Sci. 89:7422.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRPAP Antibody (AF4480)(2)

Publications using AF4480

• Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S Human Papillomavirus Type 8 Interferes with a Novel C/EBPβ-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration. PLoS Pathog., 2012-07-26;8(7):e1002833. 2012-07-26 [PMID: 22911498] (Western Blot, Human)
• Basak JM, Verghese PB, Yoon H, Kim J, Holtzman DM Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Abeta uptake and degradation by astrocytes. J. Biol. Chem., 2012-03-01;287(17):13959-71. 2012-03-01 [PMID: 22383525] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LRPAP1
• Uniprot:
  • P55302()