LRP-5 Antibody (2B11) - BSA Free

Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)

Flow Cytometry: LRP-5 Antibody (2B11) [NBP2-37510] - Flow cytometric analysis of HeLa cells using LRP5 mouse mAb (green) and negative control (red).

ELISA: LRP-5 Antibody (2B11) [NBP2-37510] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 2B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LRP-5 Antibody (2B11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human LRP-5 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRP5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 179 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LRP-5 Antibody (2B11) - BSA Free

• BMND1
• BMND1OPTA1
• EVR1
• EVR4
• HBM
• low density lipoprotein receptor-related protein 5
• low density lipoprotein receptor-related protein 7
• low-density lipoprotein receptor-related protein 5
• LR3VBCH2
• LRP5
• LRP-5
• LRP7
• LRP7exudative vitreoretinopathy 1
• OPPG
• OPS
• OPTA1
• osteoporosis pseudoglioma syndrome
• VBCH2

Background

LRP5 is involved in the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. Defects in LRP5 are a cause of autosomal dominant and autosomal recessive familial exudative vitreoretinopathy (FEVR). Autosomal dominant FEVR is also referred to as exudative vitreoretinopathy 1 (EVR1); also known as Criswick-Schepens syndrome. FEVR is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. FEVR is reported to have a penetrance of 100%, but clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP5