LRIG3 Antibody (814834) [Unconjugated]

Images

 
Western blot shows lysates of KATO‑III human gastric carcinoma cell line, HEK293T human embryonic kidney cell line, and B16‑F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
KATO‑III human gastric carcinoma cell line was stained with Mouse Anti-Human LRIG3 Monoclonal Antibody (Catalog # MAB3495, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
814834
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

LRIG3 Antibody (814834) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRIG3
Asp28-Thr807
Accession # Q6UXM1
Specificity
Detects human LRIG3 in direct ELISAs and Western blots. Detects mouse LRIG3 in Western blots. Mouse LRIG3 detection in Flow Cytometry was not tested. In direct ELISAs, no cross-reactivity with recombinant human LRIG1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LRIG3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRIG3 Antibody (814834) [Unconjugated]

  • FLJ90440
  • KIAA3016
  • leucine-rich repeats and immunoglobulin-like domains 3
  • leucine-rich repeats and immunoglobulin-like domains protein 3
  • LIG-3
  • LIG3FLJ26573
  • LIRG3
  • LRIG3

Background

LRIG3 (leucine-rich repeats and Ig-like domains-3) is an approximately 140-170 kDa type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family. This family contains three members who share 45 - 50% amino acid (aa) identity (1). All members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions, and three C2-type Ig-like domains in their extracellular domains (ECD) (1). LRIG3 mRNA is widely expressed, with highest levels in stomach, skin, thyroid and small intestine (1). Human LRIG3 is synthesized as a 1120 amino acid (aa) precursor. It contains a 24 aa signal sequence, a 786 aa ECD, a 21 aa transmembrane sequence, and a 289 aa intracellular region. One splice variant exists that has a 19 aa substitution for the first 79 aa of the standard (or long) form. This substitution appears to encode an alternate signal sequence, resulting in a mature protein that lacks the first and part of the second LRR. LRIG1, a related family member, is known to bind the EGF family receptors ErbB1-4, via either its LRR or Ig-like domains. It also binds the ubiquitin ligase, c-Cbl, and promotes ubiquitination, internalization and destruction of these receptors (2, 3). It is not known whether LRIG3 performs similar functions. Within the cell, LRIG3 is expressed in the perinuclear region as well as on the cell surface. Perinuclear location of LRIG3 in grade III and IV astrocytic tumors has been associated with better patient survival (4). Human LRIG3 ECD shares 91%, 92%, 95% and 98% aa sequence identity with mouse, rat, bovine and canine LRIG3 ECD, respectively.

  1. Guo, D. et al. (2004) Genomics 84:157.
  2. Gur, G. et al. (2004) EMBO J. 23:3270.
  3. Laederich, M.B. (2004) J. Biol. Chem. 279:47050.
  4. Guo, D. et al. (2006) Acta Neuropathol. (Berl.) 111:238.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LRIG3
Uniprot