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LRIG3 Antibody (814834) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
814834
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3495U-100UG
Alexa Fluor 405
FAB3495V-100UG
Alexa Fluor 488
FAB3495G-100UG
Alexa Fluor 532
FAB3495X
Alexa Fluor 594
FAB3495T-100UG
Alexa Fluor 647
FAB3495R-100UG
Alexa Fluor 700
FAB3495N-100UG
Alexa Fluor 750
FAB3495S-100UG
Biotin
FAB3495B
DyLight 350
FAB3495D
DyLight 405
FAB3495E
DyLight 488
FAB3495K
DyLight 550
FAB3495L
DyLight 594
FAB3495M
DyLight 650
FAB3495W
DyLight 680
FAB3495Y
DyLight 755
FAB3495Z
HRP
FAB3495H
Janelia Fluor 549
FAB3495I
Janelia Fluor 646
FAB3495J
PE
FAB3495P
PerCP
FAB3495C
Unconjugated
MAB3495
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3495
Carrier Free
MAB3495
Carrier Free
MAB3495-SP
Carrier Free
FAB3495U-100UG
Carrier Free
FAB3495T-100UG
Carrier Free
FAB3495S-100UG
Carrier Free
FAB3495R-100UG
Carrier Free
FAB3495N-100UG
Carrier Free
FAB3495G-100UG
Carrier Free
FAB3495V-100UG
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LRIG3 Antibody (814834) [FITC] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRIG3
Asp28-Thr807
Accession # Q6UXM1
Specificity
Detects human LRIG3 in direct ELISAs and Western blots. Detects mouse LRIG3 in Western blots. Mouse LRIG3 detection in Flow Cytometry was not tested. In direct ELISAs, no cross-reactivity with recombinant human LRIG1 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LRIG3
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Alternate Names for LRIG3 Antibody (814834) [FITC]

  • FLJ90440
  • KIAA3016
  • leucine-rich repeats and immunoglobulin-like domains 3
  • leucine-rich repeats and immunoglobulin-like domains protein 3
  • LIG-3
  • LIG3FLJ26573
  • LIRG3
  • LRIG3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
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EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis using 293T lysates expressing PARP. Image from verified customer review.
Western Blot: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, using this antibody. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.
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APE Antibody
(13B8E5C2) - BSA Free
NB100-116
Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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(PC10)
NB500-106
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Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
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AF3688
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NBP2-38600
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BSA Free
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Immunohistochemistry: OGG1 Antibody [NB100-106] - Figure 5 IHC-P analysis of hOGG1 protein expression in serous ovarian cancer: (A) Normal ovarian tissue treated with anti-hOGG1 antibody, biotinylated anti-rabbit IgG secondary antibody, and avidin-biotin-peroxidase complex, and counterstained with p-dimethylaminobenzaldehyde reagent (magnification 100 x); (B) serous cystadenoma with positive staining (magnification 200 x); (C) LG-SOC tissue with moderate positive staining (magnification 200 x); and (D) HG-SOC with negative staining (magnification 200 x). Black arrow pointed at the immunostained epithelial cells. Image collected and cropped by CiteAb from the following publication (http://ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-6-74), licensed under a CC-BY licence.
Immunohistochemistry: OGG1 Antibody [NB100-106] - The H&E staining ((a)-(c)) and IHC staining for oxidative DNA damage, 8-oxo-dGuo ((d)-(g)), and DNA repair enzyme hOGG1 ((h)-(j)) in control subjects and tumor and nontumor lesions of BCC patients. Values given are mean +/- SD. The statistical significance of differences between the control and case and between adjacent epidermis and tumor lesions of BCC patients was evaluated by nonparametric variables with Kruskal-Wallis test followed by Dunnett's post hoc test. * P < 0.05, *P < 0.01, *P < 0.001 compared to control; ##P < 0.01, ###P < 0.001 compared to tumor lesions of BCC patients. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/omcl/2016/5934024/), licensed under a CC-BY licence.
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Immunocytochemistry/Immunofluorescence: LRIG2 Antibody [NBP1-93621] - Staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
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POLG2 Antibody
NBP2-94064
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Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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DNA Ligase I
Antibody (10H5)
NB100-119
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Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
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Applications: ICC/IF, IHC-P, IP, In vitro, RIA, WB
EGF [Unconjugated]
236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
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XPD Antibody
AF3296
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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XPF Antibody
(OTI4E11)
NBP2-01020
Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.
Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
MYH Antibody (4D10)
H00004595-M01
Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Knockdown Validated: MYH Antibody (4D10) [H00004595-M01] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. To investigate the knockdown efficiency at protein level, western blot analysis was performed after 96 hrs of Dox treatment of cells with shRNA set1 (d). Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. The graphs represent mean +/-s.e.m. from three independent experiments. P-values were calculated using one-way analysis of variance (ANOVA). *P<0.05, **P<0.01, ***P<0.001, NS, not significant. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/oncsis201672) licensed under a CC-BY licence.
Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.
(10 Publications)
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Applications: ELISA, ICC/IF, IHC, IHC-P, IP, KD, WB
ERCC1 Antibody
(8F1) - BSA Free
NB500-704
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Anti-ERCC1 immunohistochemistry on whole sections of a lung squamous cell carcinoma, using Mab 8F1 and D-10 with CC1-mono and H2-60 protocols. Note increased cytosolic background with H2-60. Arrow: Necrotic centre. Arrowhead: Stromal axis. 100 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Cyto-histologic comparison of anti-ERCC1 immunoreactivity using the protocol 8F1 CC1-mono (top) or 8F1 H2-60 (bottom). Left: Pleural effusion sediment of lung adenocarcinoma. Right: thoracic lymph node. Arrow: Lymphocyte. Arrowhead: Streak of endothelial cells. Asterisk: Tumor cell cluster. 400 A- original magnification. Image collected and cropped by Citeab from the following publication (Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10. J Clin Bioinforma (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
(1 Review)
(15 Publications)
Species: ChHa, Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

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Bioinformatics

Gene Symbol LRIG3