LRAT Antibody - BSA Free

Western Blot: LRAT Antibody [NBP3-04496] - Analysis of extracts of various cell lines, using LRAT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Immunocytochemistry/Immunofluorescence: LRAT Antibody [NBP3-04496] - Analysis of MCF7 cells using LRAT antibody . Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LRAT Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2). MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LRAT Antibody - BSA Free

• EC 2.3.1.135
• LCA14
• lecithin retinol acyltransferase (phosphatidylcholine--retinolO-acyltransferase)
• lecithin retinol acyltransferase
• MGC33103
• Phosphatidylcholine--retinol O-acyltransferase

Background

LRAT is encoded by this gene is a microsomal enzyme that catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester, an essential reaction for the retinoid cycle in visual system and vitamin A status in liver. Mutations in this gene have been associated with early-onset severe retinal dystrophy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRAT Antibody (NBP3-04496)(1)

Publication using NBP3-04496

• IO Shmarakov, GA Gusarova, MN Islam, M Marhuenda-, J Bhattachar, WS Blaner Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice Nature Communications, 2023-02-15;14(1):851. 2023-02-15 [PMID: 36792627]

Bioinformatics

• Gene Symbol: LRAT