LOX Antibody


Western Blot: LOX Antibody [NBP2-24877] - Western blot analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NBP2-24877] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877] - Analysis of LOX in formalin-fixed, paraffin-embedded mouse skin using an isotype control (top) and NBP2-24877 (bottom) at 1:100.
Western Blot: LOX Antibody [NBP2-24877] - Analysis of LOX in A) human skin and B) mouse ear skin lysate using NBP2-24877 at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

LOX Antibody Summary

Within the range of amino acids 305-338 of human LOX protein were used as the immunogen.
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
100 ul of crude rabbit sera with 50% glycerol
0.025% Sodium Azide


  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Read Publications using
NBP2-24877 in the following applications:

Reactivity Notes

This antibody is reactive to Chimpanzee. Porcine reactivity reported in scientific literature (PMID: 26224524). Sheep reactivity reported in scientific literature (PMID: 26563105)

Alternate Names for LOX Antibody

  • lysyl oxidaseEC
  • MGC105112
  • protein-lysine 6-oxidase


LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LOX Antibody (NBP2-24877)(15)

We have publications tested in 4 confirmed species: Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-24877 Applications Species
Smith LR, Hammers DW, Sweeney HL, Barton ER. Increased collagen cross-linking is a signature of dystrophin-deficient muscle. Muscle Nerve. 2015 Nov 30 [PMID:26616495] (WB, Mouse) WB Mouse
Stephens EH, Nguyen TC, Blazejewski JG et al. Extracellular matrix remodeling in wound healing of critical size defects in the mitral valve leaflet. Heart Vessels. 2015 Nov 12 [PMID:26563105] (IHC-P, Sheep) IHC-P Sheep
Connell PS, Azimuddin AF, Kim SE et al. Regurgitation Hemodynamics Alone Cause Mitral Valve Remodeling Characteristic of Clinical Disease States In Vitro. Ann Biomed Eng. 2015 Jul 30 [PMID:26224524] (IHC-P, Porcine) IHC-P Porcine
Ozdemir BC, Pentcheva-Hoang T, Carstens JL et al. Depletion of Carcinoma-Associated Fibroblasts and Fibrosis Induces Immunosuppression and Accelerates Pancreas Cancer with Reduced Survival. Cancer Cell. 2014 May 21 [PMID:24856586] (IHC-Fr, Mouse) IHC-Fr Mouse
Nagaoka H, Terajima M, Yamada S et al. Alfacalcidol enhances collagen quality in ovariectomized rat bones. J. Orthop. Res. 2014 May 09 [PMID:24809324] (IHC-P, Rat) IHC-P Rat
Yamazaki Y, Mikami Y, Yuguchi M et al. Development of collagen fibres and lysyl oxidase expression in the presumptive dermis of chick limb bud. Anat Histol Embryol. 2012 Feb [PMID:21919949] (IHC-Fr) IHC-Fr
Atsawasuwan P, Mochida Y, Katafuchi M et al. A novel proteolytic processing of prolysyl oxidase. Connect Tissue Res. 2011 [PMID:21591931]
Barzilla JE, Acevedo FE, Grande-Allen KJ. Organ culture as a tool to identify early mechanisms of serotonergic valve disease. J Heart Valve Dis. 2010 Sep [PMID:21053743] (IHC-P, Porcine) IHC-P Porcine
Fleenor BS, Marshall KD, Durrant JR et al. Arterial stiffening with ageing is associated with transforming growth factor-B1-related changes in adventitial collagen: reversal by aerobic exercise. J Physiol. 2010 Oct 15 [PMID:20807791] (IHC-Fr, Mouse) IHC-Fr Mouse
Barzilla JE, McKenney AS, Cowan AE et al. Design and validation of a novel splashing bioreactor system for use in mitral valve organ culture. Ann Biomed Eng. 2010 Nov [PMID:20661646] (IHC-P, Porcine) IHC-P Porcine
Show All 15 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX Antibody (NBP2-24877) (0)

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Secondary Antibodies


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Blogs on LOX.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

Understanding DNA Recombination with Cre-Lox
Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan...  Read full blog post.

Cre/Lox: The Genomic Utility Knife
Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex...  Read full blog post.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol LOX

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