Western Blot: LOX Antibody [NBP2-24877] - Western blot analysis of mouse ear lysate when LOX antibody (NBP2-24877) was used at 1:1000. The band shown is the expected inactive 50 kD proenzyme.
Immunocytochemistry/ Immunofluorescence: LOX Antibody [NBP2-24877] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated ...read more
Immunohistochemistry-Paraffin: LOX Antibody [NBP2-24877] - Analysis of LOX in formalin-fixed, paraffin-embedded mouse skin using an isotype control (top) and NBP2-24877 (bottom) at 1:100.
Western Blot: LOX Antibody [NBP2-24877] - Analysis of LOX in A) human skin and B) mouse ear skin lysate using NBP2-24877 at 1:500. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used ...read more
Hu, Mu, Rt, Po, Bv, Pm, ShSpecies Glossary
WB, ICC/IF, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.
This antibody is reactive to Chimpanzee. Porcine reactivity reported in scientific literature (PMID: 26224524). Sheep reactivity reported in scientific literature (PMID: 26563105)
Alternate Names for LOX Antibody
lysyl oxidaseEC 126.96.36.199
LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development; and, its aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
LOX: A prime enzyme LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh... Read full blog post.
Understanding DNA Recombination with Cre-Lox Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan... Read full blog post.
Cre/Lox: The Genomic Utility Knife Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex... Read full blog post.