LOX-1/OLR1 Knockout HeLa Cell Lysate

Product Discontinued

LOX-1/OLR1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,32 bp deletion in exon 2 and 4 bp deletion in exon 2
• Gene: OLR1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for LOX-1/OLR1 Knockout HeLa Cell Lysate

• CLEC8A
• CLEC8ASLOX1
• C-type lectin domain family 8 member A
• hLOX-1
• Lectin-like oxidized LDL receptor 1
• Lectin-like oxLDL receptor 1
• Lectin-type oxidized LDL receptor 1
• LOX1
• LOX-1
• LOX1ox LDL receptor 1
• LOXIN
• OLR1
• oxidised low density lipoprotein (lectin-like) receptor 1
• oxidized low density lipoprotein (lectin-like) receptor 1
• oxidized low-density lipoprotein receptor 1
• oxidized low-density lipoprotein receptor 1, soluble form
• Ox-LDL receptor 1
• SCARE1
• scavenger receptor class E, member 1
• SR-E1

Background

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: OLR1