LOX-1/OLR1 Antibody

Western Blot: LOX-1/OLR1 Antibody [NBP2-89136] - Analysis of LOX1 on siHa cell lysate using anti-LOX1 antibody at 1/100 dilution.

Immunocytochemistry/Immunofluorescence: LOX-1/OLR1 Antibody [NBP2-89136] - Staining LOX1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: LOX-1/OLR1 Antibody [NBP2-89136] - Analysis of mouse brain tissue using anti-LOX-1/OLR1 antibody. Counter stained with hematoxylin.

Flow Cytometry: LOX-1/OLR1 Antibody [NBP2-89136] - Analysis of LOVO cells with LOX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: LOX-1/OLR1 Antibody [NBP2-89136] - Staining LOX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: LOX-1/OLR1 Antibody [NBP2-89136] - Analysis of human kidney tissue using anti-LOX1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: LOX-1/OLR1 Antibody [NBP2-89136] - Analysis of human placenta tissue using anti-LOX1 antibody. Counter stained with hematoxylin.

Product Discontinued

LOX-1/OLR1 Antibody Summary

• Immunogen: Recombinant protein within Human LOX-1/OLR1 aa 17-273 / 273. (SwissProt: P78380 Human; SwissProt: Q9EQ09 Mouse)
• Localization: Cell membrane. Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LOX-1/OLR1 Antibody

• CLEC8A
• CLEC8ASLOX1
• C-type lectin domain family 8 member A
• hLOX-1
• Lectin-like oxidized LDL receptor 1
• Lectin-like oxLDL receptor 1
• Lectin-type oxidized LDL receptor 1
• LOX1
• LOX-1
• LOX1ox LDL receptor 1
• LOXIN
• OLR1
• oxidised low density lipoprotein (lectin-like) receptor 1
• oxidized low density lipoprotein (lectin-like) receptor 1
• oxidized low-density lipoprotein receptor 1
• oxidized low-density lipoprotein receptor 1, soluble form
• Ox-LDL receptor 1
• SCARE1
• scavenger receptor class E, member 1
• SR-E1

Background

LOX 1 belongs to the C type lectin like protein super family. This unique scavenger receptor plays important roles in atherogenesis. Like other scavenger receptors, LOX 1 has a wide spectrum of physiological ligands, including OxLDL, anionic phospholipids, aged/apoptotic cells, activated platelets, and bacteria. The LOX 1 gene is a so called immediate early gene that is dynamically modulated by several factors in vitro and in vivo. LOX 1 expression is induced by stimuli such as inflammatory cytokines, OxLDL, TNF alpha, TGF beta, and ANG II in vitro, and several proatherogenic factors in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OLR1