LOX-1/OLR1 Antibody [Unconjugated] - C-terminus

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LOX-1/OLR1 Antibody [Unconjugated] - C-terminus Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LOX-1 (R&D Systems, Catalog # 1564-LX)
Arg60-Ile363
Accession # AAG44998
Specificity
Selected for its ability to block receptor-ligand interaction in a functional ELISA assay. In Western blots, approximately 5% cross‑reactivity with recombinant human LOX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OLR1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Blockade of Receptor-ligand Interaction
Publications
Read Publications using
AF1564 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LOX-1/OLR1 Antibody [Unconjugated] - C-terminus

  • CLEC8A
  • CLEC8ASLOX1
  • C-type lectin domain family 8 member A
  • hLOX-1
  • Lectin-like oxidized LDL receptor 1
  • Lectin-like oxLDL receptor 1
  • Lectin-type oxidized LDL receptor 1
  • LOX1
  • LOX-1
  • LOX1ox LDL receptor 1
  • LOXIN
  • OLR1
  • oxidised low density lipoprotein (lectin-like) receptor 1
  • oxidized low density lipoprotein (lectin-like) receptor 1
  • oxidized low-density lipoprotein receptor 1
  • oxidized low-density lipoprotein receptor 1, soluble form
  • Ox-LDL receptor 1
  • SCARE1
  • scavenger receptor class E, member 1
  • SR-E1

Background

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2 - 4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5 - 7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The mouse LOX-1 gene encodes a 363 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, triple repeats of an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (11). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6 - 8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

  1. Sawamura, T. et al. (1997) Nature 386:73.
  2. Daugherty, A. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223.
  3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  5. Jono, T. et al. (2002) FEBS Lett. 511:170.
  6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
  7. Delneste, Y. et al. (2002) Immunity 17:353.
  8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
  9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
  10. Chen, J. et al. (2003) Circ. Res. 94:370.
  11. Hoshikawa, H. et al. (1998) Biochem. Biophys. Res. Comm. 245:841.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF1564
Species: Mu
Applications: WB, B/N

Publications for LOX-1/OLR1 Antibody (AF1564)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Blocking, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
Neutralization
(3)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF1564 Applications Species
LE Yu, CL Lai, CT Lee, JY Wang Highly electronegative low-density lipoprotein L5 evokes microglial activation and creates a neuroinflammatory stress via Toll-like receptor 4 signaling J. Neurochem., 2017;0(0):. 2017 [PMID: 28444734] (Neutralization, Mouse) Neutralization Mouse
Akamatsu T, Dai H, Mizuguchi M, Goto Y, Oka A, Itoh M LOX-1 is a novel therapeutic target in neonatal hypoxic-ischemic encephalopathy. Am J Pathol, 2014;184(6):1843-52. 2014 [PMID: 24731447] (Neutralization, Mouse) Neutralization Mouse
The receptor of advanced glycation end products plays a central role in advanced oxidation protein products-induced podocyte apoptosis. Kidney Int, 2012;82(7):759-70. 2012 [PMID: 22622498] (Neutralization, Mouse) Neutralization Mouse
Leptin-deficient (ob/ob) mouse ovaries show fatty degeneration, enhanced apoptosis and decreased expression of steroidogenic acute regulatory enzyme. Int J Obes (Lond), 2012;36(8):1047-53. 2012 [PMID: 22083551] (Western Blot, Mouse) Western Blot Mouse
Murshid A, Gong J, Calderwood SK Heat shock protein 90 mediates efficient antigen cross presentation through the scavenger receptor expressed by endothelial cells-I. J. Immunol., 2010;185(5):2903-17. 2010 [PMID: 20686127] (Blocking, Mouse) Blocking Mouse
Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Atherosclerosis, 2009;204(2):405-11. 2009 [PMID: 19108834] (Western Blot, Mouse) Western Blot Mouse

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Biotin BAF1564

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Bioinformatics

Gene Symbol OLR1
Uniprot