LOX-1/OLR1 Antibody (331212) [Unconjugated]

THP‑1 human acute monocytic leukemia cell line were treated for 72 hours with 50 ng/mL PMA then stained with Mouse Anti-Human LOX‑1/OLR1 Monoclonal Antibody (Catalog # MAB1798, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clone: 331212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LOX-1/OLR1 Antibody (331212) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LOX-1/OLR1
  Ser61-Gln273
  Accession # P78380
• Specificity: Detects human LOX-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse LOX‑1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: OLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LOX-1/OLR1 Antibody (331212) [Unconjugated]

• CLEC8A
• CLEC8ASLOX1
• C-type lectin domain family 8 member A
• hLOX-1
• Lectin-like oxidized LDL receptor 1
• Lectin-like oxLDL receptor 1
• Lectin-type oxidized LDL receptor 1
• LOX1
• LOX-1
• LOX1ox LDL receptor 1
• LOXIN
• OLR1
• oxidised low density lipoprotein (lectin-like) receptor 1
• oxidized low density lipoprotein (lectin-like) receptor 1
• oxidized low-density lipoprotein receptor 1
• oxidized low-density lipoprotein receptor 1, soluble form
• Ox-LDL receptor 1
• SCARE1
• scavenger receptor class E, member 1
• SR-E1

Background

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2-4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5-7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca²⁺-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells, and dendritic cells (6-8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5-9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

1. Sawamura, T. et al. (1997) Nature 386:73. 
2. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223. 
3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649. 
4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193. 
5. Jono, T. et al. (2002) FEBS Lett. 511:170. 
6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
7. Delneste, Y. et al. (2002) Immunity 17:353.
8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
10. Chen, J. et al. (2003) Circ. Res. 94:370.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOX-1/OLR1 Antibody (MAB1798)(8)

Publications using MAB1798

• L Huang, KL Chambliss, X Gao, IS Yuhanna, E Behling-Ke, S Bergaya, M Ahmed, P Michaely, K Luby-Phelp, A Darehshour, L Xu, EA Fisher, WP Ge, C Mineo, PW Shaul SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature, 2019-04-24;0(0):. 2019-04-24 [PMID: 31019307] (Human)
• Ryoko Tsukahara, Hisao Haniu, Yoshikazu Matsuda, Tamotsu Tsukahara The AGP-PPAR gamma axis promotes oxidative stress and diabetic endothelial cell dysfunction Molecular and Cellular Endocrinology 9/1/2018 [PMID: 29355589]
• T Skarpengla, M Skjelland, XY Kong, K Skagen, S Holm, K Otterdal, CP Dahl, K Krohg-Søre, EL Sagen, V Bjerkeli, AH Aamodt, A Abbas, I Gregersen, P Aukrust, B Halvorsen, TB Dahl Increased Levels of Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 in Ischemic Stroke and Transient Ischemic Attack J Am Heart Assoc, 2018-01-12;7(2):. 2018-01-12 [PMID: 29330254] (IHC, Human)
• T Condamine, GA Dominguez, JI Youn, AV Kossenkov, S Mony, K Alicea-Tor, E Tcyganov, A Hashimoto, Y Nefedova, C Lin, S Partlova, A Garfall, DT Vogl, X Xu, SC Knight, G Malietzis, GH Lee, E Eruslanov, SM Albelda, X Wang, JL Mehta, M Bewtra, A Rustgi, N Hockstein, R Witt, G Masters, B Nam, D Smirnov, MA Sepulveda, DI Gabrilovic Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients Sci Immunol, 2016-08-05;1(2):. 2016-08-05 [PMID: 28417112] (Neutralization, Human)
• Fong O, Chan K, Leung K, Lam H, Cheung H, Leung T, Li K, Ng P Expression profile of cord blood neutrophils and dysregulation of HSPA1A and OLR1 upon challenge by bacterial peptidoglycan. J Leukoc Biol, 2013-08-28;95(1):169-78. 2013-08-28 [PMID: 23986550] (Immunoprecipitation, Western Blot, Human)
• Nowicki M, Kosacka J, Serke H, Bluher M, Spanel-Borowski K Altered sciatic nerve fiber morphology and endoneural microvessels in mouse models relevant for obesity, peripheral diabetic polyneuropathy, and the metabolic syndrome. J. Neurosci. Res., 2011-09-14;90(1):122-31. 2011-09-14 [PMID: 21919033] (IHC-Fr, Western Blot, Mouse)
• Zhang L, Liu Y, Lu XT, Wu YL, Zhang C, Ji XP, Wang R, Liu CX, Feng JB, Jiang H, Xu XS, Zhao YX, Zhang Y Traditional Chinese medication Tongxinluo dose-dependently enhances stability of vulnerable plaques: a comparison with a high-dose simvastatin therapy. Am. J. Physiol. Heart Circ. Physiol., 2009-10-02;297(6):H2004-14. 2009-10-02 [PMID: 19801495] (ICC, Rabbit)
• Vilser C, Hueller H, Nowicki M, Hmeidan FA, Blumenauer V, Spanel-Borowski K The variable expression of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and signs of autophagy and apoptosis in freshly harvested human granulosa cells depend on gonadotropin dose, age, and body weight. Fertil. Steril., 2009-04-01;93(8):2706-15. 2009-04-01 [PMID: 19342029] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: OLR1
• Uniprot:
  • P78380()