LONP1 Antibody - BSA Free

Immunohistochemistry: LONP1 Antibody [NBP3-38085] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using LONP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunoprecipitation: LONP1 Antibody [NBP3-38085] - Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug LONP1 antibody. Western blot was performed from the immunoprecipitate using LONP1 at a dilution of 1:1000.

Immunohistochemistry: LONP1 Antibody [NBP3-38085] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using LONP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Western Blot: LONP1 Antibody [NBP3-38085] - Western blot analysis of various lysates using LONP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LONP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LONP1 Antibody - BSA Free (NBP3-38085) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human LONP1 (NP_004784.2).
  
  Sequence:
  VFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LONP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LONP1 Antibody - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.53
• hLON ATP-dependent protease
• hLON
• lon peptidase 1, mitochondrial
• lon protease homolog, mitochondrial
• Lon protease-like protein
• LON
• LonHS
• LONP
• Mitochondrial ATP-dependent protease Lon
• mitochondrial lon peptidase 1
• mitochondrial lon protease-like protein
• PIM1
• protease, serine, 15
• PRSS15MGC1498
• Serine protease 15

Background

LONP1 encodes a mitochondrial matrix protein in the Lon family of ATP-dependent proteases. A similar E. coli protein regulates gene expression by targeting specific regulatory proteins for degradation. This protein binds a specific sequence in the light and heavy chain promoters of the mitochondrial genome which are involved in regulation of DNA replication and transcription. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LONP1