| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81734-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38085-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50478-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38085-100ul | |||
| BSA Free | NBP3-38085-20ul | |||
![Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LONP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LONP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Simple Western: LONP1 Antibody [NBP1-81734] - Simple Western lane view shows a specific band for LONP1 in 0.2 mg/ml of h. Kidney (left) , NIH-3T3 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LONP1-Antibody-Simple-Western-NBP1-81734-img0003.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0015.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human fallopian tube shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LONP1 Antibody [NBP1-81734] - PINK1 accumulates upon knockdown of Lon. Western blot analysis of Lon protease (Lon), Mitochondrial transcription factor A (TFAM), Heat shock protein 60 (Hsp60) and Actin in whole head homogenate from control and Lon deficient animals. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1004279), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: LONP1 Antibody [NBP1-81734] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81734-img0009.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human tonsil shows granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0018.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0020.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human pancreas shows granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0017.jpg)
![Simple Western: LONP1 Antibody [NBP1-81734] - Electropherogram image(s) of corresponding Simple Western lane view. LONP1 antibody was used at 1:20 dilution on h. Kidney and NIH-3T3 lysate(s).](http://images.novusbio.com/fullsize/LONP1-Antibody-Simple-Western-NBP1-81734-img0005.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - MTO1 defective cells exhibit proteostasis stress and an altered bioenergetic state. Representative immunoblot showing the expression of LONP1 in extracts of WT and MTO1 HF. Porin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29348686/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-81734_rabbit-polyclonal-lonp1-antibody-2092023181153.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - Altered mitochondrial features in cybrid cells carrying MELAS, MERRF, m.5514 A > G (mt-tRNATrp), m.1643A > G (mt-tRNAVal), & m.14487 T > C (ND6) mutations. (A) Representative western blot of LONP1, AFG3L2 & CLPP peptidases in mutant & wild type (WT) cybrid cells. The membrane was also probed with porin as a loading control. Full-length western blots & lower-exposure blots of porin are included in supplementary information. (B) Densitometric analysis of LONP1, AFG3L2 & CLPP normalized to porin & represented as fold change relative to WT (top). Quantitative data are from at least three independent experiments. Results from this analysis are also shown as a heatmap (bottom). The color & the corresponding value in log2 scale are depicted on the left. (C) Representative Blue Native-PAGE of OXPHOS complexes in mutant & WT cybrid cells. Full-length blots & lower-exposure blots for those with high contrast are included in supplementary information. (D) Densitometric analysis of OXPHOS complexes normalized to complex-II (loading control) & represented as fold change relative to WT. (E) Cellular ATP determination in mutant & WT cybrid cells. (F & G) Determination of Ca2+ (F) & ROS (G) by flow cytometry in mutant & WT cybrid cells with Fluo-3 & MitoSOX Red, respectively. All data are the mean ± SEM of at least three different experiments. Differences from WT values were found to be statistically significant at *p < 0.05, **p < 0.01 & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28740091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81734_rabbit-polyclonal-lonp1-antibody-31020241612215.jpg)
![Western Blot: LONP1 Antibody [H00009361-D01P] - Analysis of LONP1 expression in mouse kidney.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-H00009361-D01P-img0009.jpg "Western Blot: LONP1 Antibody [H00009361-D01P] - Analysis of LONP1 expression in mouse kidney.")
![Immunocytochemistry/Immunofluorescence: LONP1 Antibody [H00009361-D01P] - Analysis of antibody to LONP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunocytochemistry-Immunofluorescence-H00009361-D01P-img0006.jpg)
![Western Blot: LONP1 Antibody [H00009361-D01P] - Analysis of LONP1 expression in HepG2.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-H00009361-D01P-img0007.jpg)
![Western Blot: LONP1 Antibody [H00009361-D01P] - Analysis of LONP1 expression in human kidney.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-H00009361-D01P-img0008.jpg)
![Western Blot: LONP1 Antibody [H00009361-D01P] - Analysis of LONP1 expression in transfected 293T cell line by LONP1 polyclonal antibody.Lane 1: LONP1 transfected lysate(106.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-H00009361-D01P-img0010.jpg)
![Immunohistochemistry: LONP1 Antibody [NBP3-38085] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using LONP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38085_rabbit-lonp1-pab-912202415133457.jpg "Immunohistochemistry: LONP1 Antibody [NBP3-38085] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using LONP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.")
![Immunoprecipitation: LONP1 Antibody [NBP3-38085] - Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug LONP1 antibody. Western blot was performed from the immunoprecipitate using LONP1 at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38085_rabbit-lonp1-pab-91220241517527.jpg)
![Immunohistochemistry: LONP1 Antibody [NBP3-38085] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using LONP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38085_rabbit-lonp1-pab-912202415152889.jpg)
![Western Blot: LONP1 Antibody [NBP3-38085] - Western blot analysis of various lysates using LONP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38085_rabbit-lonp1-pab-912202415143658.jpg)
![Western Blot: LONP1 Antibody (9H10) [NBP3-42990] - Jurkat cells were subjected to SDS PAGE followed by western blot with (LONP1 Antibody) at dilution of 1:5000](http://images.novusbio.com/fullsize/nbp3-42990_mouse-lonp1-mab-9h10-101220241723674.jpg "Western Blot: LONP1 Antibody (9H10) [NBP3-42990] - Jurkat cells were subjected to SDS PAGE followed by western blot with (LONP1 Antibody) at dilution of 1:5000")
![Immunohistochemistry: LONP1 Antibody (9H10) [NBP3-42990] - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (LONP1 Antibody) at dilution of 1:800 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).](http://images.novusbio.com/fullsize/nbp3-42990_mouse-lonp1-mab-9h10-10122024172365.jpg)
![Western Blot: LONP1 Antibody [NBP2-76504] - Western blot analysis using Anti-LONP1 antibody NBP2-76504 (A) shows similar pattern to independent antibody NPB1-81734 (B).](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP2-76504-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: LONP1 Antibody [NBP2-76504] - Western blot analysis using Anti-LONP1 antibody NBP2-76504 (A) shows similar pattern to independent antibody NPB1-81734 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: LONP1 Antibody [NBP2-76504] - Staining of human cell line U-251 MG shows localization to nucleus & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76504-img0002.jpg)
![Western Blot: LONP1 Antibody (LON20/H1) [NBP2-50478] - U87 cells were transfected with siRNA against the Lon protease (siLonp1.1 and siLonp1.2) or a control sequence (siControl) or transfection reagent alone (mock) and then western blotted using anti-LONP1 [LON20/H1] and a loading control antibody (B-actin). Molecular weight markers are labelled in kilodaltons](http://images.novusbio.com/fullsize/LONP1-Antibody-LON20-H1-Western-Blot-NBP2-50478-img0001.jpg "Western Blot: LONP1 Antibody (LON20/H1) [NBP2-50478] - U87 cells were transfected with siRNA against the Lon protease (siLonp1.1 and siLonp1.2) or a control sequence (siControl) or transfection reagent alone (mock) and then western blotted using anti-LONP1 [LON20/H1] and a loading control antibody (B-actin). Molecular weight markers are labelled in kilodaltons")
![Western Blot:[NBP3-30286]-Detection of human LONP1/PRSS15 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LONP1/PRSS15 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30286_rabbit-lonp1-pab-23120251204041.jpg "Western Blot:[NBP3-30286]-Detection of human LONP1/PRSS15 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LONP1/PRSS15 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: LONP1 Overexpression Lysate (Adult Normal) [NBP2-07825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LONP1.](http://images.novusbio.com/fullsize/LONP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07825-img0001.jpg "Western Blot: LONP1 Overexpression Lysate (Adult Normal) [NBP2-07825] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LONP1.")
![Western Blot: LONP1 Antibody (3B2) [H00009361-M01] - detection against Immunogen (37.22 KDa) .](http://images.novusbio.com/fullsize/LONP1-Antibody-3B2-Western-Blot-H00009361-M01-img0001.jpg "Western Blot: LONP1 Antibody (3B2) [H00009361-M01] - detection against Immunogen (37.22 KDa) .")