LMP2/PSMB9 Antibody

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Western Blot: LMP2/PSMB9 Antibody [NBP1-52090] - Analysis of Proteasome 20S LMP2 in Human Thymus lysate (35ug protein in RIPA buffer) using NBP1-52090 at 1ug/ml. Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

LMP2/PSMB9 Antibody Summary

Immunogen
Peptide with sequence C-RNISYKYRED corresponding to internal region according to NP_002791.1.
Epitope
C-RNISYKYRED
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMB9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 22 kDa band observed in human thymus lysates and approx. 20 kDa band in rat thymus (calculated MW of 23.3 kDa band according to human NP_002791.1 and rat NP_036840.2).
Publications
Read Publication using NBP1-52090.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine, Porcine, Rabbit.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LMP2/PSMB9 Antibody

  • beta1i
  • LMP2
  • LMP2MGC70470
  • Low molecular mass protein 2
  • Macropain chain 7
  • Multicatalytic endopeptidase complex chain 7
  • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2)
  • proteasome catalytic subunit 1i
  • Proteasome chain 7
  • proteasome subunit beta 6i
  • proteasome subunit beta type-9
  • Proteasome subunit beta-1i
  • proteasome-related gene 2
  • PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2)
  • PSMB9
  • Really interesting new gene 12 protein
  • RING12
  • RING12EC 3.4.25.1

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding different isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LMP2/PSMB9 Antibody (NBP1-52090)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMB9
Entrez
Uniprot