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LYK5 Antibody

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Western Blot: LYK5 Antibody [NBP2-24700] - Analysis of LYK5 in A549 cell lysate in the (A) absence and (B) presence of immunizing peptide using NBP2-24700 at 1 ug/ml.
Western Blot: LYK5 Antibody [NBP2-24700] - Analysis of LYK5 in A549 cell lysate in the (A) absence and (B) presence of immunizing peptide using NBP2-24700 at 1 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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LYK5 Antibody Summary

Immunogen
A portion of amino acids 300-350 of human STRAD (isoform 1) was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STRADA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Control
LYK5 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP2-24700 in the following applications:

  • WB
    1 publication

Reactivity Notes

The amino acid sequence used is 100% homologous in Yellow Fluorescent Protein (YFP) and Cyan Fluorescent Protein (CFP). The NCBI accession number for GFP is AAC78774. Mouse reactivity reported in scientific literature (PMID: 24345663).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for LYK5 Antibody

  • LYK5FLJ90524
  • NY-BR-96
  • protein kinase LYK5
  • Serologically defined breast cancer antigen NY-BR-96
  • STE20-like pseudokinase
  • STE20-related adapter protein
  • STE20-related adaptor protein
  • STE20-related kinase adapter protein alpha
  • STE20-related kinase adaptor alpha
  • Stlk
  • STRAD alpha
  • STRADPMSE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in human testes lysate.
Immunocytochemistry/Immunofluorescence: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: LKB1 Antibody [NBP2-14835] - LKB1 antibody was tested in mouse stomach using DAB with hematoxylin counterstain.
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CAB39 Antibody
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Western Blot: CAB39 Antibody [NBP2-15658] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: CAB39 Antibody [NBP2-15658] - CAB39 antibody detects CAB39 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CAB39 protein stained by CAB39 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658] - Paraffin-embedded mouse testis. CAB39 antibody diluted at 1:500.
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
AMPK alpha 2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.
Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.
Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.
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Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
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Western Blot: PAWR / PAR4 Antibody [NBP1-87338] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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Western Blot: MST3 Antibody [NBP1-87833] - Analysis using Anti-STK24 antibody NBP1-87833 (A) shows similar pattern to independent antibody NBP1-87834 (B).
Immunocytochemistry/Immunofluorescence: MST3 Antibody [NBP1-87833] - Staining of human cell line A-431 shows localization to nucleoli & cytosol. Antibody staining is shown in green.
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Western Blot: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC1.
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Western Blot: TSC1 Antibody (3A2) [NBP2-46234] - Analysis of extracts (10ug) from 2 different cell lines.
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Western Blot: SLC12A3 Antibody [NBP1-44270] - analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody at 1:1000.
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GM130/GOLGA2
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Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody [NBP2-53420] - HeLa cells were fixed for 10 min using 10% formalin and then permeabilized for 5 min using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GOLGA2 at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: GM130/GOLGA2 Antibody [NBP2-53420] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-GOLGA2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP2-53420] - Analysis of a FFPE tissue section of mouse intesting using GM130/GOLGA2 antibody at 1:1500. The antibody generated staining in the the Golgi apparatus in the perinuclear region.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
CRM1 Antibody
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Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. (B) The effect of LMB treatment on the cellular localization of Nup98-HoxA9. Nup98-HoxA9 ES cells were cultured either in the presence or absence of 5 nM LMB for 2 hr, fixed and stained with antibodies against FLAG (M2) and CRM1. Merged images of FLAG (green) and CRM1 (red) are shown. Nuclei were stained with DAPI. Bar, 10um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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ALS2CR2 Antibody
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Western Blot: ALS2CR2 Antibody [NBP2-24680] - analysis of Als2Cr2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse brain using Als2Cr2 antibody at 4.0 ug/ml and 2 ug/ml, respectively.
Immunohistochemistry-Paraffin: ALS2CR2 Antibody [NBP2-24680] - Formalin-fixed, paraffin-embedded human kidney stained with Als2Cr2 antibody at 20 ug/ml. Tubule staining can be seen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Applications: WB, IHC, IHC-P
MARK1 Antibody
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Western Blot: MARK1 Antibody [NBP1-33409] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: MARK1 Antibody [NBP1-33409] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Species: Hu
Applications: WB, ICC/IF
MST4 Antibody
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Western Blot: MST4 Antibody [NB100-1004] - Staining of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MST4 Antibody [NB100-1004] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
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(3B12)
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Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in NIH/3T3.
Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in PC-12.
Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in A-431.
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
TSC2 Antibody
(614204) [Unconjugate
MAB40401
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSC2 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TSC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
TSC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu, Mu
Applications: WB, IHC, ICC
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Publications for LYK5 Antibody (NBP2-24700)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Control Lysate(s)

LYK5 Knockout HeLa Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol STRADA