LIS1 Antibody (5A5) - Azide and BSA Free

Western Blot: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of PAFAH1B1 expression in transfected 293T cell line by PAFAH1B1 monoclonal antibody (M03), clone 5A5. Lane 1: PAFAH1B1 transfected lysatE (46.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: LIS1 Antibody (5A5) [H00005048-M03] - Analysis of monoclonal antibody to PAFAH1B1 on HeLa cell. Antibody concentration 15 ug/ml

Sandwich ELISA: LIS1 Antibody (5A5) [H00005048-M03] - Detection limit for recombinant GST tagged PAFAH1B1 is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Po, Bv, Sh
• Applications: WB, ELISA, ICC/IF
• Clone: 5A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LIS1 Antibody (5A5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PAFAH1B1 (NP_000421, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSP
• Specificity: PAFAH1B1 - platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa (5A5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAFAH1B1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIS1 Antibody (5A5) - Azide and BSA Free

• LIS-1
• LIS1PAF-AH alpha
• LIS2
• lissencephaly 1 protein
• Lissencephaly-1 protein
• MDCR
• MDS
• Miller-Dieker syndrome chromosome region
• PAF acetylhydrolase 45 kDa subunit
• PAF-AH 45 kDa subunit
• PAFAH alpha
• PAFAH
• PAFAHA
• platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• platelet-activating factor acetylhydrolase IB subunit alpha
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Background

PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIS1 Antibody (H00005048-M03)(1)

Publication using H00005048-M03

• Wong CH, Wingett SW, Qian C et Al. Genome-scale requirements for dynein-based trafficking revealed by a high-content arrayed CRISPR screen bioRxiv 2023-03-01 [PMID: 36909483]

Bioinformatics

• Gene Symbol: PAFAH1B1
• Entrez:
  • 5048(Human)
• Uniprot:
  • NP_000421(Human)