LIMPII/SR-B2 Overexpression Lysate (Native)

Images

 
Western Blot: SR-BII Overexpression Lysate (Adult Normal) [NBP2-04294] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SR-BII.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LIMPII/SR-B2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_005497. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SR-BII, mRNA.
Gene
SCARB2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LIMPII/SR-B2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LIMPII/SR-B2 Overexpression Lysate (Native)

  • AMRF
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
  • CD36 antigen
  • CD36L2
  • CD36L2LIMP II
  • HLGP85
  • LGP85
  • LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
  • LIMPII
  • LIMPIICD36 antigen-like 2
  • LPG85
  • lysosome membrane protein 2
  • Lysosome membrane protein II
  • SCARB2
  • Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
  • scavenger receptor class B, member 2
  • SRB2
  • SR-B2
  • SR-BII

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-104
Species: Hu, Mu, Rt, ChHa, SyHa, Ha, Md, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB120-19294
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22217
Species: Hu, Mu, Rt(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
H00002629-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
AF1029
Species: Mu
Applications: WB, IHC, IP
NBP2-38049
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
H00000777-R01
Species: Hu
NBP1-88370
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
NB100-56605
Species: Av, Bv, Sh
Applications: WB
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF4146
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-04294
Species: Hu
Applications: WB

Publications for LIMPII/SR-B2 Lysate (NBP2-04294) (0)

There are no publications for LIMPII/SR-B2 Lysate (NBP2-04294).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LIMPII/SR-B2 Lysate (NBP2-04294) (0)

There are no reviews for LIMPII/SR-B2 Lysate (NBP2-04294). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LIMPII/SR-B2 Lysate (NBP2-04294) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional LIMPII/SR-B2 Products

Bioinformatics Tool for LIMPII/SR-B2 Lysate (NBP2-04294)

Discover related pathways, diseases and genes to LIMPII/SR-B2 Lysate (NBP2-04294). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LIMPII/SR-B2 Lysate (NBP2-04294)

Discover more about diseases related to LIMPII/SR-B2 Lysate (NBP2-04294).
 

Pathways for LIMPII/SR-B2 Lysate (NBP2-04294)

View related products by pathway.

PTMs for LIMPII/SR-B2 Lysate (NBP2-04294)

Learn more about PTMs related to LIMPII/SR-B2 Lysate (NBP2-04294).
 

Research Areas for LIMPII/SR-B2 Lysate (NBP2-04294)

Find related products by research area.

Blogs on LIMPII/SR-B2

There are no specific blogs for LIMPII/SR-B2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SR-BI Antibody
NB400-104

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LIMPII/SR-B2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SCARB2
Entrez