LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free

Western Blot: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM Kinas

Immunocytochemistry/Immunofluorescence: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1.

Immunohistochemistry: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM Kinase 1 mouse monoclonal antibody.

Flow Cytometry: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LIM Kinase 1 antibody, and then analyzed by flow cytometry.

Western Blot: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LIM Kinase 1 monoclonal antibody.

Immunohistochemistry: LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free [NBP2-71141] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIM Kinase 1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free (NBP2-00748) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIMK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 72.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for LIM Kinase 1 Antibody (OTI3H6) - Azide and BSA Free

• EC 2.7.11.1
• LIM domain kinase 1
• LIM Kinase 1
• LIM motif-containing protein kinase
• LIMK
• LIMK1
• LIMK-1
• LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIMK1