Lgr6 Antibody (8J2J10)

Western Blot: Lgr6 Antibody (8J2J10) [NBP3-16758] - Western blot analysis of extracts of various cell lines, using Lgr6 Rabbit mAb (NBP3-16758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunocytochemistry/Immunofluorescence: Lgr6 Antibody (8J2J10) [NBP3-16758] - Immunofluorescence analysis of HeLa cells using Lgr6 Rabbit mAb (NBP3-16758) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: Lgr6 Antibody (8J2J10) [NBP3-16758] - Western blot analysis of extracts of Rat testis, using Lgr6 Rabbit mAb (NBP3-16758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 8J2J10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Lgr6 Antibody (8J2J10) Summary

• Description: Novus Biologicals Rabbit Lgr6 Antibody (8J2J10) (NBP3-16758) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 801-900 of human Lgr6 (Q9HBX8). FPVTPEAVKSVLLVVLPLPACLNPLLYLLFNPHFRDDLRRLRPRAGDSGPLAYAAAGELEKSSCDSTQALVAFSDVDLILEASEAGRPPGLETYGFPSVT
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LGR6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Lgr6 Antibody (8J2J10)

• FLJ14471
• gonadotropin receptor
• GPCR
• leucine-rich repeat containing G protein-coupled receptor 6
• leucine-rich repeat-containing G protein-coupled receptor 6
• leucine-rich repeat-containing G-protein coupled receptor 6
• Lgr6
• VTS20631

Background

LGR6 has been reported to be expressed in rat adrenal, brain, colon, heart, kidney, ovary, oviduct, small intestine, spleen, testis, and uterus Hsu et al., 2000). ESTs have been isolated from human B-cell/lung/testis, blood, brain, colon, eye, germ cell, head/neck, heart, heart/melanocyte/uterus, lung, ovary, stomach, testis, and uterus libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGR6