MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB8458) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8458-SP | |||
| Carrier Free | FAB8458P-025 | |||
| Carrier Free | FAB8458V-100UG | |||
| Carrier Free | FAB8458U-100UG | |||
| Carrier Free | FAB8458T-100UG | |||
| Carrier Free | FAB8458S-100UG | |||
| Carrier Free | FAB8458R-100UG | |||
| Carrier Free | FAB8458N-100UG | |||
| Carrier Free | FAB8458G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26169-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22172-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-49696 | |||
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NLS442] - Brain, Amygdala](http://images.novusbio.com/fullsize/Lgr6-Antibody-Immunohistochemistry-Paraffin-NLS442-img0007.jpg "Immunohistochemistry-Paraffin: Lgr6 Antibody [NLS442] - Brain, Amygdala")
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NLS442] - Analysis of anti-LGR6 antibody with human brain, neuron at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Lgr6-Antibody-Immunohistochemistry-Paraffin-NLS442-img0004.jpg)
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NLS442] - Skin, melanoma](http://images.novusbio.com/fullsize/Lgr6-Antibody-Immunohistochemistry-Paraffin-NLS442-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NLS442] - Brain, Alzheimer's disease, neuritic plaque](http://images.novusbio.com/fullsize/Lgr6-Antibody-Immunohistochemistry-Paraffin-NLS442-img0006.jpg)
![Western Blot: Lgr6 Antibody (JE39-78) [NBP3-32400] - Western blot analysis of Lgr6 on different lysates with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (10 ug/Lane)<br/> Lane 2: MCF7 cell lysate (10 ug/Lane)<br/> Lane 3: SKOV-3 cell lysate (10 ug/Lane)<br/> Lane 4: Mouse testis tissue lysate (20 ug/Lane)<br/><br/> Predicted band size: 104 kDa<br/> Observed band size: 104 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32400_rabbit-lgr6-mab-je39-78-27820241524268.jpg "Western Blot: Lgr6 Antibody (JE39-78) [NBP3-32400] - Western blot analysis of Lgr6 on different lysates with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/1,000 dilution.<br/><br/> Lane 1: HeLa cell lysate (10 ug/Lane)<br/> Lane 2: MCF7 cell lysate (10 ug/Lane)<br/> Lane 3: SKOV-3 cell lysate (10 ug/Lane)<br/> Lane 4: Mouse testis tissue lysate (20 ug/Lane)<br/><br/> Predicted band size: 104 kDa<br/> Observed band size: 104 kDa<br/><br/> Exposure time: 30 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lgr6 Antibody (JE39-78) [NBP3-32400] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32400) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32400_rabbit-lgr6-mab-je39-78-298202414161874.jpg)
![Immunocytochemistry/ Immunofluorescence: Lgr6 Antibody (JE39-78) [NBP3-32400] - Immunocytochemistry analysis of HeLa cells labeling Lgr6 with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Lgr6 antibody (NBP3-32400) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32400_rabbit-lgr6-mab-je39-78-2920241114822.jpg)
![Western blot analysis of rat testis tissue lysates with NBP3-32400 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32400 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32400_-western-blot-639204597796143849.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
1324-WN)." alt="Recombinant Human Lgr6 induces Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2.5-12.5 µg/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # ![Immunohistochemistry: Lgr6 Antibody (15G3) [NBP3-26169] - Image of Lgr6 Antibody (15G3) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-262202414471575.jpg "Immunohistochemistry: Lgr6 Antibody (15G3) [NBP3-26169] - Image of Lgr6 Antibody (15G3) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.")
![Immunocytochemistry/Immunofluorescence: Lgr6 Antibody (15G3) [NBP3-26169] - Staining of hela with Lgr6 Antibody (15G3) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 500-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-28220241312228.jpg)
![Flow Cytometry: Lgr6 Antibody (15G3) [NBP3-26169] - Overlay Peak curve showing Hela cells stained with Lgr6 Antibody (15G3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-282202414183610.jpg)
![Western Blot: Lgr6 Antibody (8J2J10) [NBP3-16758] - Western blot analysis of extracts of various cell lines, using Lgr6 Rabbit mAb (NBP3-16758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Lgr6-Antibody-8J2J10-Western-Blot-NBP3-16758-img0002.jpg "Western Blot: Lgr6 Antibody (8J2J10) [NBP3-16758] - Western blot analysis of extracts of various cell lines, using Lgr6 Rabbit mAb (NBP3-16758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Lgr6 Antibody (8J2J10) [NBP3-16758] - Immunofluorescence analysis of HeLa cells using Lgr6 Rabbit mAb (NBP3-16758) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lgr6-Antibody-8J2J10-Immunocytochemistry-Immunofluorescence-NBP3-16758-img0001.jpg)
![Western Blot: Lgr6 Antibody (8J2J10) [NBP3-16758] - Western blot analysis of extracts of Rat testis, using Lgr6 Rabbit mAb (NBP3-16758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Lgr6-Antibody-8J2J10-Western-Blot-NBP3-16758-img0003.jpg)
![Western Blot: Lgr6 Antibody (SR1033) [NBP3-22172] - Western blot analysis using NBP3-22172 in HUVEC cell lysate.](http://images.novusbio.com/fullsize/nbp3-22172_rabbit-lgr6-mab-sr1033-57202316582193.jpg "Western Blot: Lgr6 Antibody (SR1033) [NBP3-22172] - Western blot analysis using NBP3-22172 in HUVEC cell lysate.")
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NBP3-14391] - Immunohistochemistry of formalin-fixed, paraffin-embedded human neutrophils after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Lgr6-Antibody-Immunohistochemistry-Paraffin-NBP3-14391-img0001.jpg "Immunohistochemistry-Paraffin: Lgr6 Antibody [NBP3-14391] - Immunohistochemistry of formalin-fixed, paraffin-embedded human neutrophils after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Lgr6 Antibody [NBP3-14391] - Immunohistochemistry of formalin-fixed, paraffin-embedded human spleen after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Lgr6 Antibody-Immunohistochemistry-Paraffin-NBP3-14391-img0002.jpg)
![Western Blot: LGR6 Overexpression Lysate (Adult Normal) [NBP2-11018] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LGR6.](http://images.novusbio.com/fullsize/LGR6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11018-img0001.jpg "Western Blot: LGR6 Overexpression Lysate (Adult Normal) [NBP2-11018] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LGR6.")
![Western Blot: LGR6 Overexpression Lysate (Adult Normal) [NBP2-11261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LGR6.](http://images.novusbio.com/fullsize/LGR6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11261-img0001.jpg "Western Blot: LGR6 Overexpression Lysate (Adult Normal) [NBP2-11261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LGR6.")
