Lgr5/GPR49 Antibody (750835) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 750835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Lgr5/GPR49 Antibody (750835) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line, CHO derived recombinant human Lgr5/GPR-49
  Met1-Ile560
  Accession # O75473
• Specificity: Detects human Lgr5/GPR-49 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lgr5/GPR49 Antibody (750835) [Unconjugated]

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

GPR49 (G-protein-coupled receptor 49), also called LGR5 (leucine-rich repeat GPR 5) is a seven-transmembrane glycoprotein receptor that negatively regulates of Wnt signaling in the developing intestine. Expression of GPR49 is upregulated in intestinal stem cells and intestinal cancer stem cells and promotes carcinogenesis. GPR49 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (aa 22-561) with 16 LRR domains. Human GPR49 shares 90% aa sequence identity with mouse and rat GPR45 within aa 22-561.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O75473(Human)