LEF1 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEF1 at approximately 35-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LEF1 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Goat Anti-Human LEF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7647) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LEF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LEF1
  Met120-Met280 (predicted)
  Accession # Q9UJU2
• Specificity: Detects human LEF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TCF-1, rhTCF-2, and rhTCF-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LEF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LEF1 Antibody [Unconjugated]

• DKFZp586H0919
• FLJ46390
• LEF1
• LEF-1
• lymphoid enhancer-binding factor 1
• T cell-specific transcription factor 1-alpha
• TCF10
• TCF1ALPHA
• TCF1-alpha
• TCF7L3

Background

LEF1 (Lymphoid Enhancer-binding Factor 1) is a functionally diverse member of the TCF/LEF family of transcription factors. The name LEF1 was originally applied to the mouse factor, while its human counterpart was named TCF-1 alpha . Since there is a related molecule named TCF-1, to avoid confusion with TCF-1 alpha , both species factors are now called LEF1. Although its predicted MW is 44 kDa, it runs anomalously at 35-55 kDa in SDS-PAGE. LEF1 has restricted expression in adult, being limited to T cells, thymocytes, pre-B cells and NK cells. LEF1 demonstrates sequence-specific (CCTTTG[T/A][T/A]) DNA binding and directs beta -catenin to Wnt‑responsive genes. Either transcriptional activation or repression may occur on LEF1 target genes, depending upon 1) the cofactors recruited to the LEF1: beta ‑catenin complex, and 2) the phosphorylation state of LEF1. Human LEF1 is 399 amino acids (aa) in length. It contains one DNA-binding HMG-box (aa 298‑369), three potential phosphorylation sites, and an N-terminal SUMOylation site at Lys27. There are at least five isoform variants. One utilizes an alternative start site at Met116, a second contains a 47 aa substitution for aa 283‑299, and a third combines the previous two variations. A fourth variant shows a deletion of aa 214‑241, while a fifth variant combines the afore mentioned deletion of aa 214-241 with a 25 aa substitution for aa 390‑399. Over aa 120‑280, human LEF1 shares 97% aa sequence identity with mouse LEF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LEF1 Antibody (AF7647)(2)

Publications using AF7647

• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020-04-07;31(1):107471. 2020-04-07 [PMID: 32268087] (ICC, Human)
• Y Luo, R Ge, H Wu, X Ding, H Song, H Ji, M Li, Y Ma, S Li, C Wang, H Du The osteogenic differentiation of human adipose-derived stem cells is regulated through the let-7i-3p/LEF1/beta-catenin axis under cyclic strain Stem Cell Res Ther, 2019-11-21;10(1):339. 2019-11-21 [PMID: 31753039] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: LEF1
• Uniprot:
  • Q9UJU2(Human)