Rat LDLR ELISA Kit (Colorimetric)

ELISA: Rat LDLR ELISA Kit (Colorimetric) [NBP2-81149] - Samples were spiked with high concentrations of Rat LDLR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat LDLR ELISA Kit (Colorimetric) [NBP2-81149] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Rat LDLR ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat LDLR in samples. No significant cross-reactivity or interference between Rat LDLR and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.53%
• Intra-Assay: CV% < 4.87%
• Spike Recovery: 89-109%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LDLR

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat LDLR ELISA Kit (Colorimetric)

• FH
• FHC
• LDL R
• LDL receptor
• LDLCQ2
• LDLR
• low density lipoprotein receptor
• low-density lipoprotein receptor class A domain-containing protein 3
• low-density lipoprotein receptor

Background

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis. Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits. It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) were shown to be mediated by LDL receptors on cultured cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LDLR